Acta Chimica Sinica ›› 2010, Vol. 68 ›› Issue (17): 1719-1725. Previous Articles     Next Articles

Full Papers

三聚氰胺与鯡鱼精DNA相互作用研究

沈昊宇*,朱凡,施炜,陈真,潘青青   

  1. (浙江大学宁波理工学院生物与化工分院 宁波 315100)
  • 收稿日期:2009-08-18 修回日期:2010-01-07 出版日期:2010-09-14 发布日期:2010-05-06
  • 通讯作者: 沈昊宇 E-mail:haoyushen2000@163.com
  • 基金资助:

    浙江省科技基础条件平台研究项目(2007F0045);宁波市自然科学基金资助项目(2009A610002)

Studies of Interaction between Melamine and Herring Sperm DNA

SHEN Hao-Yu, ZHU Fan, SHI Wei, CHEN Zhen, PAN Qing-Qing   

  1. (Ningbo Institute of Technology, Zhejiang University, Ningbo 315100)
  • Received:2009-08-18 Revised:2010-01-07 Online:2010-09-14 Published:2010-05-06
  • Contact: Shen Hao-Yu Shen E-mail:haoyushen2000@163.com

UV-Vis spectroscopy, fluorescence spectroscopy, cyclic voltametry (CV), and viscosimetric methods were applied to investigate the mechanism of the interaction between melamine (MM) and herring sperm DNA. The effect of MM on the migration rate of pBR322 plasmid DNA was investigated by gel electrophoretic method as well. The results showed that for the system of pH at 7.0, the charge transfer coefficients (α) were 0.27 and 0.26, with the velocity constant (ks) at 5.58 s-1 and 5.65 s-1 for the MM before and after DNA added, respectively. The interaction between MM and DNA may be realized via groove combined mode. The thermodynamic formulation constant (K) was ca. 105 mol-1•L.

Key words: melamine, herring sperm DNA, interaction mode, dynamic constant, thermodynamic formulation constant