Acta Chimica Sinica ›› 2010, Vol. 68 ›› Issue (18): 1864-1870. Previous Articles     Next Articles

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  1. (1山西大学分子科学研究所 化学生物学与分子工程教育部重点实验室 太原 030006)
    (2山西大学化学化工学院 太原 030006)
  • 投稿日期:2010-04-12 修回日期:2010-05-13 发布日期:2010-05-24
  • 通讯作者: 杨频
  • 基金资助:


Study on the Interaction between Epirubicin-Fe2+ System and DNA by Spectroscopic and Electrochemical Methods

Xi Xiaoli1 Yang Manman2 Yang Pin*,1   

  1. (1 Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education of China at College of Chemistry and Chemical Engineering, Institute of Molecular Science, Shanxi University, Taiyuan 030006)
    (2 College of Chemistry and Chemical Engeneering, Shanxi University, Taiyuan 030006)
  • Received:2010-04-12 Revised:2010-05-13 Published:2010-05-24

Epirubicin is used to treat the fast multiplication cancer. The interaction of Epirubicin-Fe2+ system with calf thymus DNA has been investigated using UV spectra, fluorescent spectra, CD, viscosity and cyclic voltammetry etc. The results show that Epirubicin can form constant system with Fe2+ in pH=7.4 solution. Hypochromism was observed in the UV spectra of the system in the presence of the DNA. The Scatchard plots showed competitive inhibition aganist EB binding to DNA. The relative viscosity and thermal deformation temperature of DNA increased with addition of the Epirubicin-Fe2+ system. The fluorescence polarization of the DNA-EB system increased with addition of the Epirubicin-Fe2+ system. Determination of cyclic voltammetry showed that the DNA made the Epirubicin-Fe2+ formal potential positively shift. The energy of base pair has been weakened with addition of the Epirubicin-Fe2+ system. So it can be concluded that the binding mode of the Epirubicin-Fe2+ system with CT DNA belongs to intercalation action. This work may be usefully applied to elucidating the mechanisms of natural nucleases and drug design.

Key words: Epirubicin-Fe2+ system, calf thymus DNA, Scatchard plot