Acta Chimica Sinica ›› 2011, Vol. 69 ›› Issue (05): 543-547. Previous Articles     Next Articles

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  1. (1兰州军区兰州总医院临床药理基地 兰州 730050)
    (2兰州大学生命科学学院 兰州 730000)
    (3兰州大学药学院 兰州 730000)
    (4西北师范大学生命科学学院 兰州 730070)
  • 投稿日期:2010-05-17 修回日期:2010-06-07 发布日期:2010-10-26
  • 通讯作者: 王荣
  • 基金资助:


Capillary Electrophoresis Detection of Lung Cancer and Adjacent Normal Tissue Differences in Protein Mixture

Liu Yong1,2 Wang Rong*,1,2,3 Gao Lan2 Jia Zhengping1,2 Xie Hua1 Zhang JunLi1 Ma Jun1 Zhang Aimei4 Xie Xihui3   

  1. (1 Department of Pharmacy, Lanzhou General Hospital of the Chinese People s Liberty Army, Lanzhou 730050)
    (2 Life Science College, Lanzhou University, Lanzhou 730000)
    (3 Pharmaceutical School, Lanzhou University, Lanzhou 730000)
    (4 Life Science College, Northwest Normal University, Lanzhou 730070)
  • Received:2010-05-17 Revised:2010-06-07 Published:2010-10-26
  • Contact: Rong Wang

Protein mixture (denatured and native) differences of extracted from lung cancer and adjacent normal tissue by established method of capillary electrophoresis (CE)-laser-induced fluorescence (LIF) detection of protein was detected using the fluorescein isothiocyanate (FITC) as a derivative agent. When detected by the 1×TBE electrophoresis buffer solution (pH 10.0 of denaturing gel electrophoresis and pH 8.3 of native gel electrophoresis containing 2 mg/L Coomassie brilliant blue), separation voltage of 15 kV, column temperature of 15 ℃, electric injection (10 kV×10 s), and excitation wavelength/emission wavelength of 488/520 nm, lung cancer and adjacent normal tissue protein mixture samples obtained a better separation and there was significant difference. Comparing with the current commonly used analysis methods, the SDS-denatured polyacrylamide gel electrophoresis (SDS-PAGE) as well as the Blue Native polyacrylamide gel electrophoresis (BN-PAGE), the BN-PAGE results showed that compared to normal tissue, the lung cancer tissue had more significant protein type differences. But the SDS-PAGE results showed that some differences of protein expression had significant difference between the lung cancer and adjacent normal tissue, and mainly concentrated in 20~116 kDa. CE-LIF detection results were roughly the same with PAGE, and CE-LIF is more sensitivey than PAGE and it can more accurately reflect protein differences of lung cancer and adjacent normal tissue. We concluded that the CE-LIF could be used to detect protein differences. And the time was shorter, effect was better, and analysis of active protein reflected its advantage of providing a strong driving force-voltage and a well temperature stability by strong motivation.

Key words: proteomics, capillary electrophoresis, lung cancer, protein mixture, polyacrylamide gel electrophoresis, blue native polyacrylamide gel electrophoresis