Acta Chimica Sinica ›› 2011, Vol. 69 ›› Issue (05): 601-604. Previous Articles    




  1. (1江苏省生物功能材料重点实验室 南京师范大学化学与材料科学学院 南京210097)
    (2江南大学工业生物技术教育部重点实验室 无锡 214122)
  • 投稿日期:2010-10-21 修回日期:2010-11-30 发布日期:2010-12-02
  • 通讯作者: 黄晓华
  • 基金资助:


Effect of Heavy Metal Cadmium Ion on Outward Potassium Channel in Cell

Du Chunlei1 Wang Lihong1,2 Jiang Na1 Huang Xiaohua*,1   

  1. (1 Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097)
    (2 The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122)
  • Received:2010-10-21 Revised:2010-11-30 Published:2010-12-02
  • Supported by:

    the Natural Science Foundation of China

Cadmium (Cd) contamination leads to a variety of clinical diseases. Potassium ion (K) channel is the most diverse and ubiquitous class of ion channels, and it plays the important physiological roles in human. In order to elucidate the cellular mechanism of the pathogenic and toxic effect of Cd2+pollution, the effects of Cd2+on the currents of a human ether-a-go-go related gene (hERG) Kchannel was investigated by a whole-cell patch clamp method. The results indicated that Cd2+ significantly inhibited the steady-state and tail currents of hERG Kchannel. When cells were treated with 10, 50, 200 μg/L Cd2+, the activation curve of hERG Kchannel was right-shifted, and the slope factor was not changed. The currents of hERG Kchannel was not significantly changed with increasing the concentration of Cd2+. The results suggested that potassium channels can be used as the target of the research on the the cellular mechanism of the pathogenic and toxic effect of Cd2+pollution.

Key words: cadmium, hERG, potassium ion channel, HEK293

CLC Number: