Acta Chimica Sinica ›› 2011, Vol. 69 ›› Issue (09): 1101-1106. Previous Articles     Next Articles

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滇桂艾纳香多糖BRP的结构解析

许子竞,林翠梧*   

  1. (广西大学化学化工学院 南宁 530004)
  • 收稿日期:2010-08-20 修回日期:2010-10-21 出版日期:2011-05-14 发布日期:2010-12-13
  • 通讯作者: 林翠梧 E-mail:lincuiwu@126.com
  • 基金资助:

    国家自然科学基金项目

Structure Identification of Polysaccharide BRP from Blumea Riparia

XU Zi-Jing, LIN Cui-Wu   

  1. (College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004)
  • Received:2010-08-20 Revised:2010-10-21 Online:2011-05-14 Published:2010-12-13
  • Contact: Cui-wu LIN E-mail:lincuiwu@126.com

A polysaccharide (BRP) was obtained from Blumea riparia, by hot water extraction, deproteination using Sevag method, precipitation with ethanol, and fractionation through DEAE-cellulose column, Sephadex G-200 and Sepharose 6 F.F. Gel filtration chromatography. Based on UV and IR spectrum, HPLC, HPGPC and GC-MS chromatography, NMR (1H NMR, 13C NMR, HMQC, HMBC) technology and methylation reaction, the structure of BRP was identified. The results showed that BRP was hightly purified with average molecular weight of 3.3×104 Da determinted by HPGPC. And it was composed of Fru by HPLC analysis. The backbone of BRP had a consisting of 1,2-linked β-D-fructofuranosyl residues. Finally, the structure of BRP was fixed on as: β-D-Fruf-(2[→1)-β-D-Fruf(2→]n-1)-β-D-Fruf.

Key words: polysaccharide (BRP), isolation, NMR, methylation, structure characterization