Acta Chimica Sinica ›› 2005, Vol. 63 ›› Issue (11): 1003-1007. Previous Articles     Next Articles

Original Articles


庄贵生1,刘菁1,贾春平1,金庆辉1,赵建龙*1 2,王惠民3   

  1. (1中国科学院上海微系统与信息技术研究所 上海 200050)
    (2生物芯片上海国家工程研究中心 上海 201203)
    (3南通大学附属医院 南通 226001)
  • 投稿日期:2004-07-14 修回日期:2005-02-21 发布日期:2010-12-10
  • 通讯作者: 赵建龙

Manufacture of Reversible Electrophoresis Chip and Its Application to Protein Detection

ZHUANG Gui-Sheng1, LIU Jing1, JIA Chun-Ping1, JIN Qing-Hui1, ZHAO Jian-Long*1,2, WANG Hui-Min3   

  1. (1 Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050)
    (2 National Engineering Center for Biochip at Shanghai, Shanghai 201203)
    (3 Affiliated Hospital of Nantong University, Nantong 226001)
  • Received:2004-07-14 Revised:2005-02-21 Published:2010-12-10
  • Contact: ZHAO Jian-Long

This paper described a low-cost and reliable process for reversible bonding fabrication of electrophoresis microchips on quartz glass substrates in ordinary laboratory without the requirement of clean room facilities, and the microchips have been used in model protein separation and clinical urinary protein detection. Patterns of microchannel were fabricated through standard photolithography and etching on the quartz glass substrate. After bonding surfaces were rigorously rinsed, bonding was realized in vacuum ovens. The chip could be separated and re-bonded for usage. The microchip in these studies was designed to realize on-chip separation of urinary proteins with UV detection. The electrophoretic buffer was 75 mmol/L borate with 0.5 mol/L lactate and pH 10.5. The chips have been used successfully for separation of model proteins and clinical urinary proteins.

Key words: reversible bonding, electrophoresis microchip, urinary protein