Acta Chimica Sinica ›› 2011, Vol. 69 ›› Issue (04): 445-451. Previous Articles     Next Articles

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  1. (1大连理工大学精细化工国家重点实验室 大连 116024)
    (2大连理工大学生命科学与技术学院 大连 116024)
  • 投稿日期:2010-07-14 修回日期:2010-08-26 发布日期:2010-10-21
  • 通讯作者: 彭孝军
  • 基金资助:


The Spectral Properties of Cy5 with Aldehyde Group Substituted at Meso-position of the Polymethine Chain Utilized as Fluorescent Viscosity Sensor

Yang Zhigang1 He Yanxia1 Dai Bin1 Dou Bairui2 Wang Jingyun2 Peng Xiaojun*,1   

  1. (1 State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024)
    (2 School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024)
  • Received:2010-07-14 Revised:2010-08-26 Published:2010-10-21

Compared with the traditional cyanine dyes (Cy5), both the maximum absorption and emission wavelengths of new Cy5 dyes with aldehyde group substituted at central position of the conjugated chain slightly blue shift, and the Stokes Shift is enlarged evidently to more than 28 nm, and largest up to 48 nm in water. The fluorescence quantum yield is rather low without obvious change in different solvents. It is found that the fluorescence of dye is sensitive to viscosity of the solvents, and the emission intensity of the dye can be enhanced largely by ascending of environmental viscosity with well linear relationship between lg If and lg (viscosity) (F?rster-Hoffmann equation). It is living cell membrane permeable for the dye 4 to map cellular microenvironmental viscosity, which demonstrates the potential application for dye 4 to investigate the intracellular microviscosity change.

Key words: cyanine dyes, Cy5, viscosity, fluorescent sensor, cell imaging