Acta Chimica Sinica ›› 2012, Vol. 70 ›› Issue (12): 1401-1406.DOI: 10.6023/A1112061 Previous Articles     Next Articles

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赵常志, 赵改爽, 张兆霞, 梁俊钰   

  1. 青岛科技大学化学与分子工程学院 山东青岛 266042
  • 投稿日期:2011-12-06 修回日期:2012-03-20 发布日期:2012-03-20
  • 通讯作者: 赵常志
  • 基金资助:

    山东省自然科学基金(No. 2009ZRB01393)和电分析化学国家重点实验室开放基金(No. SKLEAC201106)资助项目.

Preparation and Application of Photoelectrochemical Guanine Sensor

Zhao Changzhi, Zhao Gaishuang, Zhang Zhaoxia, Liang Junyu   

  1. College of Chemistry & Molecular Engineering, Qingdao University of Science & Technology, Qingdao 266042, China
  • Received:2011-12-06 Revised:2012-03-20 Published:2012-03-20
  • Supported by:

    Project supported by the Natural Science Foundation of Shandong Province (No. 2009ZRB01393) and Electroanalytical Chemistry State Key Laboratory Fund (No. SKLEAC201106).

We report herein a new guanine sensor for the determination of guanine based on a photoelectrochemical effect of poly(thionine) photoelectrode to hydrogen peroxide (H2O2). The photoelectrode was fabricated by electropolymerizing thionine on the surface of ITO electrode. And then xanthine oxidase was immobilized on the photoelectrode via the aid of chitosan enwrapping, forming an enzyme-modified photoelectrode. The photoelectrode was employed as an electron acceptor, and H2O2 from the catalytic reaction of enzyme was employed as an electron donor, the guanine can be detected due to photoelectrochemical effect between photoelectrode and H2O2. In the paper, photoelectrochemical effects of photoelectrode to H2O2 and guanine were studied. Effects of bias voltage, illumination intensity and electrolyte pH on the photocurrent were investigated. The linear response of guanine concentrations ranged from 1.00~200 μmol/L was obtained with a detection limit of 0.55 μmol/L. The guanine sensor was successfully applied to detect the disorganization of DNA mediated by heating acid solution and active ingredient of aciclovir. The relative standard deviation (RSD) was less than 5.37% and the recovery was in the range of 96.8%~106% for the determination of samples. The preparation of the sensor and the detection of guanine have the advantages of economy and simpleness due to without peroxidase and deoxygenated.

Key words: guanine sensor, photoelectrochemistry, guanine, xanthine oxidase, herring sperm DNA, aci clovir