Acta Chimica Sinica ›› 1982, Vol. 40 ›› Issue (12): 1159-1165. Previous Articles     Next Articles

有机氟键合相高效液体色谱填料的研究——Ⅳ.1,1,2,2-四氢-7-三氟甲基十二氟辛基键合相填料

李文华, 王锦华, 陈立佛   

  1. 中国科学院上海有机化学研究所
  • 收稿日期:1981-09-23 出版日期:1982-12-15 发布日期:2013-06-03
  • 通讯作者: 李文华

STUDIES ON ORGANIC FLUORO-BONDED PHASE AS HPLC PACKING Ⅳ. 1, 1, 2, 2-Tetrahydro-7-trifluoromethyldodecafluorooxtyl Bonded Phase Packing

LI WEN-HUA, WANG JIN-HUA, CHEN LI-FO   

  1. Shanghai Institute of Organic Chemistry, Academia Sinica
  • Received:1981-09-23 Online:1982-12-15 Published:2013-06-03

A flnoroalkylbonded phase used.as HPLC packing has been prepared from silica (5μ) and 1, 1, 2, 2-tetrahydro-7 trifluoromethyldodecaflnorooctyltrichloro-ailane, (CF3)2CF (CF2)4CH2CH2SiCL3.By controlling the surface concentration of the organic ligand, we prepared the packing which may be need either in normal phase or in reverse phase mode.Comparing with the perfluoroamide bonded phase packing previously prepared by us, it not only has the same chromatographio performance to give a much shorter retention time for polar ateroidal compounds, to improve the peak symmetry and reduce tailing, bnt also more hydrophobic and leas polar in nature.This fluoroalkyl bonded phase packing gives a good separation in nonaqneons reverse phase mode towards some choleric acids which formerly can only be separatedtion exchange or reverse phase chromatography and show severe tailing on ciliagel column.On the other hand, the separation of phenols and polyhydroxy steroids are also anccesaful on thin column in reverse phase mode.