有机化学 ›› 2019, Vol. 39 ›› Issue (9): 2485-2491.DOI: 10.6023/cjoc201903010 上一篇    下一篇

研究论文

一种新型连续检测镉离子和焦磷酸阴离子的逻辑门荧光传感器及其细胞成像研究

常永新a, 李白a, 郭淼b, 蔡永红a, 徐括喜a   

  1. a 河南大学化学化工学院 功能有机分子研究中心 河南省阻燃与功能材料工程实验室 开封 475004;
    b 河南大学纳米材料工程研究中心 开封 475004
  • 收稿日期:2019-03-05 修回日期:2019-03-18 出版日期:2019-09-25 发布日期:2019-04-11
  • 通讯作者: 蔡永红, 徐括喜 E-mail:caiyonghong@henu.edu.cn;xukx@henu.edu.cn
  • 基金资助:

    国家自然科学基金(No.U1404207)资助项目.

A Novel Logic Gate Fluorescent Sensor for the Sequential Detection of Cd2+ and Pyrophosphate Anion and Application in Cell Imaging

Chang Yongxina, Li Baia, Guo Miaob, Cai Yonghonga, Xu Kuoxia   

  1. a Engineering Laboratory for Flame Retardant and Functional Materials of Henan Province, Institute of Functional Organic Molecular Engineering, College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004;
    b Institute of Nanomaterials Engineering Research Center, Henan University, Kaifeng 475004
  • Received:2019-03-05 Revised:2019-03-18 Online:2019-09-25 Published:2019-04-11
  • Contact: 10.6023/cjoc201903010 E-mail:caiyonghong@henu.edu.cn;xukx@henu.edu.cn
  • Supported by:

    Project supported by the National Natural Science Foundation of China (No. U1404207).

设计并合成了一种基于8-羟基喹啉(8-HQ)的连续检测Cd2+和焦磷酸阴离子(PPi)的荧光传感器L.在传感器L的DMSO/H2O(V/V=1/1,0.01 mol/L,Hepes-HCl buffer,pH=7.20)溶液中加入Cd2+后导致荧光发射峰(Em=537 nm)猝灭,检测限低至5.87×10-8 mol/L.通过Job's曲线图和质谱验证传感器L和Cd2+离子之间以1:1化学计量比结合,结合常数为4.38×104 L/mol.复合传感器L-Cd2+体系具有通过配体置换法对PPi高度选择性检测性能.L可以作为一种逻辑门荧光传感器检测Cd2+和PPi.传感器L可以用于对活细胞中的Cd2+和PPi的荧光成像.

关键词: 8-羟基喹啉, 荧光传感器, 连续检测, 活细胞成像

A novel fluorescent sensor L based on 8-hydroxyquinoline (8-HQ) has been designed and synthesized. The sensor L displayed excellent sensitivity specific toward Cd2+ and pyrophosphate anion (PPi), respectively. The fluorescence emission peak at 537 nm was quenched when Cd2+ was added to the DMSO/H2O (V/V=1/1, 0.01 mol/L, Hepes-HCl buffer, pH=7.20) solution of sensor L and the detection limit was as low as 5.87×10-8 mol/L. The 1:1 stoichiometry was verified by Job's plot and mass spectrum, and the association constant was obtained to be 4.37×104 L/mol. The further study found that the L-Cd2+ system has high selectivity for detection of PPi by ligand replacement method and L could be used as a logic gate fluorescence sensor. In addition, the sensor L was employed for imaging the Cd2+ and PPi in living cells.

Key words: 8-hydroxyquinoline, fluorescent sensor, sequential detection, cell imaging