Chin. J. Org. Chem. ›› 2018, Vol. 38 ›› Issue (9): 2243-2258.DOI: 10.6023/cjoc201806001 Previous Articles     Next Articles

Special Issue: 合成科学

Reviews

单萜吲哚生物碱的合成生物学研究进展

吴世文a, 杨盟权a,b, 肖友利a,b   

  1. a 中国科学院上海生命科学研究院植物生理生态研究所/分子植物科学卓越创新中心 中国科学院合成生物学重点实验室 上海 200032;
    b 中国科学院大学 北京 100039
  • 收稿日期:2018-06-01 修回日期:2018-08-04 出版日期:2018-09-25 发布日期:2018-08-14
  • 通讯作者: 肖友利 E-mail:ylxiao@sibs.ac.cn
  • 基金资助:

    上海市科学技术委员会(Nos.18ZR1447000,15JC1400402)资助项目.

Synthetic Biology Studies of Monoterpene Indole Alkaloids

Wu Shiwena, Yang Mengquana,b, Xiao Youlia,b   

  1. a CAS Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032;
    b University of Chinese Academy of Sciences, Beijing 100039
  • Received:2018-06-01 Revised:2018-08-04 Online:2018-09-25 Published:2018-08-14
  • Contact: 10.6023/cjoc201806001 E-mail:ylxiao@sibs.ac.cn
  • Supported by:

    Project supported by the Science and Technology Commission of Shanghai Municipality (Nos. 18ZR1447000, 15JC1400402).

Monoterpene indole alkaloids (MIAs) are a group of important specialized metabolites that exhibit a broad range of pharmacological activities. However, the contents of MIAs in natural plants are extremely low. It could be produced through advanced synthetic chemistry, but the production scale is very limited, and the further modification of the key scaffold is very difficult. With the full elucidation of MIAs biosynthetic pathway and deeper investigation of biotechnology in synthetic biology, the use of synthetic biology strategies combined with chemical semi-synthesis will be an important development trend for the synthesis of these compounds and their derivatives. Elucidation of MIAs biosynthetic pathway by characterizing involved genes is the prerequisite for synthetic biology. Strictosidine, which was formed by the combination of tryptamine and secologanin, is the key intermediate for MIAs biosynthesis. The discovery of biological parts and research advances of synthetic biology of MIAs based on the upstream biosynthetic pathway, the formation of strictosidine, and downstream biosynthetic pathway, production of divergent MIAs derived from strictosidine over the past three decades are described. This review could provide guidance for further elucidation of other MIAs and promotion of synthetic biology for producing MIAs.

Key words: monoterpene indole alkaloid, strictosidine, biological part, biosynthetic pathway, synthetic biology