^a Jiangsu Key Laboratory for Chemistry of Low-dimensional Materials, Huaiyin Normal University, Huai'an, Jiangsu 223300, China 
^b Department of Chemistry, College of Science, Yanbian University, Yanji, Jilin 133002, China

The direct electrochemistry of glucose oxidase (GOD) was achieved based on the immobilization of GOD on a natural nano-structural attapulgite (ATP) clay film modified glassy carbon (GC) electrode. The immobilized GOD displayed a pair of well-defined quasi-reversible redox peaks with a formal potential (E0') of －457.5 mV (vs. SCE) in 0.1 mol•L－1 pH 7.0 phosphate buffer solution. The peak current was linearly dependent on the scan rate, indicating that the direct electrochemistry of GOD in that case was a surface-controlled process. The immobilized glucose oxidase could retain bioactivity and catalyze the oxidation of glucose in the presence of ferrocene monocarboxylic acid (FMCA) as a mediator with the apparent Michaelis-Menten constant of 1.16 mmol•L－1. The electrocatalytic response showed a linear dependence on the glucose concentration ranging widely from 5.0×10－6 to 6.0×10－4 mol•L－1 (with correlation coefficient of 0.9960). This work demonstrated that the nano-structural attapulgite clay was a good candidate material for the direct electrochemistry of the redox-active enzyme and the construction of the related enzyme biosensors. The proposed biosensors were applied to determine the glucose in blood and urine samples with satisfactory results.