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Chinese Journal of Chemistry 2009, 27(12) 2413-2417 DOI:
ISSN: 1001-604X CN: 31-1547/O6 |
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Synthesis of 2-(1H-Benzo[d]imidazol-2-yl)phenyl Phosphate as a Fluorescent Probe for Determination of ALP |
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LEI Yingjie*; OUYANG Jie*; ZHANG Youlai; DING Mei |
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School of Chemistry & Chemical Engineering, Tianjin University of Technology, Tianjin 300384 |
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Abstract：
A convenient method to synthesize 2-(1H-benzo[d]imidazol-2-yl)phenyl phosphate (HBIPP), a substrate for the fluorescent determination of alkaline phosphatase (ALP) activity, via the reaction of salicylaldehyde and o-phenylenediamine in the presence of manganese(III) acetate was realized. The structure was confirmed by IR, 1H NMR, EI-MS and elemental analysis studies while the corresponding photo-physical properties of the enzyme reaction product were characterized by ultraviolet absorption and fluorescence spectra in methanol. Preliminary tests reveal that the amount of the enzyme reaction product, 2-(2-hydroxyphenyl) benzimidazole (HPBI), is proportional to ALP activity in the range of 0.01—5 U/L with the detection limit of 8.5 mU/L, which can be applied to assess ALP in biomedical research. |
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Keywords：
alkaline phosphatase
fluorescent probe
2-(1H-benzo[d]imidazol-2-yl)phenyl phosphate
synthesis
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Received 2009-03-04 Revised 2009-06-01 Online: 2009-07-23 |
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Email: lyj@tjut.edu.cn |
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