Acta Chimica Sinica ›› 2011, Vol. 69 ›› Issue (24): 2981-2986.DOI: 10.6023/A1104171 Previous Articles     Next Articles

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反射模式与线性模式MALDI-TOF MS联合分析荔枝果核缩合单宁

周海超*, 林益明*, 柴纬明, 魏淑东, 廖蒙蒙   

  1. (滨海湿地生态系统教育部重点实验室 厦门大学生命科学学院 厦门 361005)
  • 投稿日期:2011-04-17 修回日期:2011-07-31 发布日期:2011-10-11
  • 通讯作者: 林益明
  • 基金资助:


Characterization of Condensed Tannins from Litchi Seed by Reflec-tron Modes and Linear Modes of MALDI-TOF MS

ZHOU Hai-Chao, LIN Yi-Ming, CHAI Wei-Ming, WEI Shu-Dong, LIAO Meng-Meng   

  1. (Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005)
  • Received:2011-04-17 Revised:2011-07-31 Published:2011-10-11

Condensed tannins from litchi seed were analyzed using reflectron modes and linear modes of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The reflectron modes (accelerating voltage, 20.0 kV|reflectron voltage, 23.0 kV|delayed extraction voltage, 16.32 kV|lens voltage, 9.45 kV) spectra obtained clear information on basic unit, interflavan linkage and polymerization degree extended from 3-mers to 18-mers. The linear modes (accelerating voltage, 20.0 kV|delayed extraction voltage, 16.25 kV|lens voltage, 10.0 kV) spectra further expanded the detectable polymers up to 27-mers. Litchi seed condensed tannins were A-type procyanidins polymer with as high as 27-mers. In the present study, reflectron modes coupled with linear modes was discussed as a potential method of MALDI-TOF MS in characterization of condensed tannins.

Key words: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, reflectron mode, linear mode, litchi seed, condensed tannins