Acta Chimica Sinica ›› 2012, Vol. 70 ›› Issue (03): 352-356.DOI: 10.6023/A1104252 Previous Articles     Next Articles

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柯楠因诱导寡聚腺嘌呤核苷酸双螺旋结构增强赛博绿I 荧光及柯楠因的高灵敏和高选择荧光检测

周亚文a, 李春梅a, 熊祖洪b, 黄承志a,c   

  1. a 西南大学化学化工学院 发光与实时分析教育部重点实验室 重庆 400715;
    b 西南大学物理科学与技术学院 重庆 400715;
    c 西南大学药学院 重庆 400716
  • 投稿日期:2011-04-25 修回日期:2011-09-27 发布日期:2011-10-25
  • 通讯作者: 黄承志
  • 基金资助:

    国家重大新药创制(No. 2010ZX09401-306-1-4)、国家自然科学基金(No. 21035005)和西南大学基本科研业务费专项基金项目(No. XDJK2009A001)资助项目.

Investigations on the Enhanced Fluorescence of Sybr Green I by Coralyne-induced Double-stranded Structure of Adenine Oligonucleotides and a Sensitive and Selective Spectroflurometry of Coralyne

Zhou Yawena, Li Chunmeia, Xiong Zuhongb, Huang Chengzhia,c   

  1. a Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715;
    b College of Physics and Technology, Southwest University, Chongqing 400715;
    c College of Pharmaceutical Sciences, Southwest University, Chongqing 400716
  • Received:2011-04-25 Revised:2011-09-27 Published:2011-10-25
  • Supported by:

    Project was supported by the National Creating New Drug Program of China (No. 2010ZX09401-306-1-4), National Natural Science Foundation of China (No. 21035005) and Scientific Research Foundation of Southwest University (No. XDJK2009A001).

Coralyne is one of the protoberberine alkaloids analogues. It was found that coralyne could induce adenine oligonucleotides (poly A16) to form a double strand DNA under quasi-physiological conditions, greatly enhancing the fluorescence of Sybr Green I (SG I), a DNA intercalative dye. With the enhanced fluorescence signals of SG I, a new method for the determination of coralyne has been developed in linear range of 60~600 nmol/L, with the detection limit (3σ) of 30 nmol/L. This proposed method is highly selective and other analogues have no interfering effects on the determination, which has been successfully applied for analysis of synthetic samples with the recovery from 92.5% to 105%, and the RSD lower than 3.1%.

Key words: coralyne, adenine oligonucleotides, Sybr Green I, fluorescence spectrometry