Acta Chimica Sinica ›› 2012, Vol. 0 ›› Issue (05): 649-658 .DOI: 10.6023/A1105024 Previous Articles     Next Articles

Full Papers


吴丽君a, 段佳a, 李倩倩a, 曹金莉b, 刘玮a, 闵顺耕a   

  1. a 中国农业大学理学院应用化学系 北京 100193;
    b 云南省烟草公司大理州公司 云南 671000
  • 投稿日期:2011-05-02 修回日期:2011-10-10 发布日期:2011-11-28
  • 通讯作者: 闵顺耕
  • 基金资助:

    云南省烟草公司(No. 09YN016)资助项目.

Identification of the Volatile Components in Tobacco by Gas Chromatograph Coupled with Ion Trap Tandem Mass Spectrometry

Wu Lijuna, Duan Jiaa, Li Qianqiana, Cao Jinlib, Liu Weia, Min Shungenga   

  1. a Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193;
    b Tobacco Corporation of Yunnan Dali, Yunnan 671000
  • Received:2011-05-02 Revised:2011-10-10 Published:2011-11-28
  • Supported by:

    Project supported by Tobacco Corporation of Yunnan Dali (No. 09YN016).

The volatile components in tobacco leaves were extracted by simultaneous distillation and extraction (SDE), and analyzed by gas chromatography-mass spectrometry (GC-MS). The volatile components in tobacco leaves were identified by library searching and spectral matching combined with retention index. Ion trap tandem mass spectrometry was introduced to identify some components which had tiny difference of library matching, low concentration, and complex matrix. 144 components were identified by library searching, spectral matching and ion trap tandem mass spectrometry in this paper. And 104 volatile components have been reported, 9 of them were identified by ion trap tandem mass spectrometry, the other 40 components have not been reported in the literature. So ion trap tandem mass spectrometry is the precise and reliable method to identify some unknown components, and is suitable for separation and identification of complex system.

Key words: simultaneous distillation and extraction, tobacco, volatile components, ion trap tandem mass spectrometry