Acta Chimica Sinica ›› 2012, Vol. 70 ›› Issue (07): 843-851 .DOI: 10.6023/A1110262 Previous Articles     Next Articles

Full Papers


陈丽娜a,b, 宋凤瑞a, 郑重a, 邢俊鹏a, 刘志强a, 刘淑莹a   

  1. a 中国科学院长春应用化学研究所 长春质谱中心 长春 130022;
    b 中国科学院研究生院 北京 100049
  • 投稿日期:2011-10-26 修回日期:2011-11-30 发布日期:2012-01-05
  • 通讯作者: 刘志强
  • 基金资助:

    “十一五”国家科技支撑计划(No. 2009ZX09308-006)和国家科技部创新方法工作专项(No. 2010IM030400)资助项目.

Studies on the Determination Method of Pesticide Multi-residues in Ginseng by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry

Chen Linaa,b, Song Fengruia, Zheng Zhongaa, Xing Junpenga, Liu Zhiqianga, Liu Shuyinga   

  1. a Changchun Institute of Applied Chemistry Chinese Academy of Sciences, Changchun Centre of Mass Spectrometry, Changchun 130022;
    b Graduate School of Chinese Academy of Sciences, Beijing 100049
  • Received:2011-10-26 Revised:2011-11-30 Published:2012-01-05
  • Supported by:

    Project supported by the National Key Technology R&D Program in the 11th Five year Plan of China (No. 2009ZX09308-006) and Innovation Method Fund of China (No. 2010IM030400).

Taking the traditional herbal, ginseng, as the object in the paper, a comprehensive analytical method based on matrix solid-phase dispersion-ultra performance liquid chromatography tandem mass spectrometry (MSPD-UPLC-MS/MS) has been developed for the simultaneous determination of 46 pesticide residues in Ginseng. The sample was prepared by MSPD. Primary secondary amine (PSA) was used as dispersant, and acetonitrile was used as eluant. Both positive and negative ionization modes were used in the detection. Qualitative and quantitative analysis was carried out by UPLC-MS/MS under multiple reaction monitoring mode (MRM). The linear range of this method was 5~500.0 μg/kg. The limits of quantification (LOQ) for the 46 pesticides were less than 0.01 mg/kg. The mean recoveries at the three spiked levels of 10~100 μg/kg were 70%~110%, with relative standard deviations (RSD) better than 15% (n=3). The method is robust, selective, sensitive, rapid and cheap.

Key words: matrix solid-phase dispersion, ultra performance liquid chromatography tandem mass spectrometry, ginseng, multi-residue pesticides