Acta Chimica Sinica ›› 2012, Vol. 70 ›› Issue (11): 1283-1288.DOI: 10.6023/A1201124 Previous Articles     Next Articles

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碳量子点对基于能量转移电化学发光DNA传感体系影响的研究

杨帆, 王伶俐, 郭志慧   

  1. 陕西省生命分析化学重点实验室 陕西师范大学化学化工学院 西安 710062
  • 收稿日期:2012-01-12 修回日期:2012-02-27 出版日期:2012-06-14 发布日期:2012-02-27
  • 通讯作者: 郭志慧
  • 基金资助:

    国家自然科学基金(No. 20905044)资助项目.

Study on the Influence of Carbon Quantum Dots on Electrogenerated Chemiluminescence Energy Transfer Sensing System of DNA

Yang Fan, Wang Lingli, Guo Zhihui   

  1. Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062
  • Received:2012-01-12 Revised:2012-02-27 Online:2012-06-14 Published:2012-02-27
  • Supported by:

    Project supported by the National Natural Science Foundation of China (No. 20905044).

The effect of carbon quantum dots on the electrogenerated chemiluminescence (ECL) energy transfer sensing system for the determination of DNA was investigated. The results showed that the signal- to-noise ratio of luminol-crystal violet ECL system was increased and its reproducibility was improved by the addition of carbon quantum dots. The conditions of the detection of DNA were optimized. Under optimal conditions, the linear range for the determination of target DNA was 1.0×10-11~1.0×10-7 mol/L, the correlation coefficient was 0.9881 and the detection limit was 4.0×10-12 mol/L. The possible interaction mechanism between DNA and carbon quantum dots was also discussed. This method was convenient and showed high sensitivity.

Key words: electrogenerated chemiluminescence, carbon quantum dots, DNA detection, luminol, crystal violet