Acta Chim. Sinica ›› 2015, Vol. 73 ›› Issue (1): 29-35.DOI: 10.6023/A14110805 Previous Articles     Next Articles



王大伟a, 林红艳a, 曹润洁a, 杨盛刚a, 陈涛a, 何波a, 陈琼a, 杨文超a, 杨光富a,b   

  1. a 华中师范大学化学学院 教育部农药与化学生物学重点实验室 武汉 430079;
    b 天津化学化工协同创新中心 天津 300071
  • 投稿日期:2014-11-22 发布日期:2015-01-04
  • 通讯作者: 陈琼, 杨光富;
  • 基金资助:

    项目受国家"十二五"科技支撑计划(No. 2011BAE06B03)资助和国家自然科学基金面上项目(Nos. 21372093, 21172091)资助.

Synthesis and Bioactivity Studies of Triketone-Containing Quinazoline-2,4-dione Derivatives

Wang Daweia, Lin Hongyana, Cao Runjiea, Yang Shengganga, Chen Taoa, He Boa, Chen Qionga, Yang Wenchaoa, Yang Guangfua,b   

  1. a Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079;
    b Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300071
  • Received:2014-11-22 Published:2015-01-04
  • Supported by:

    Project supported by the National Key Technologies R&D Program of China (No. 2011BAE06B03) and the National Natural Science Foundation of China (Nos. 21372093, 21172091).

4-Hydroxyphenylpyruvate dioxygenase (EC, HPPD) is an important enzyme in the catabolism of tyrosine, catalyzing the conversion of 4-hydroxyphenyl pyruvic acid (HPPA) into homogentisic acid (HGA), belonging to the 2-his-1-carboxylate facial triad family of non-heme iron(II) enzymes. As an important enzyme in regulating the biosynthesis of tocopherols and plastoquinone in plants, HPPD is an important target for herbicides discovery. Previously, we have found that triketone-containing quinazoline-2,4-dione motif can be used as a novel lead structure for herbicides discovery. In this continuous work, we synthesized a total number of 24 new triketone-containing quinazoline-2,4-dione derivatives. The new compounds 9a9x were prepared by using 5-methyl-2-nitrobenzoic acid as the starting material, followed by oxidation, esterification and reduction reactions, the main intermediate dimethyl 4-aminoisophthalate could be obtained in a yield of 83%, then by another six steps of reactions the target compounds could be smoothly synthesized. All the title compounds were characterized by 1H NMR, 13C NMR and HRMS spectrum data. To explore the biology activity of these compounds, their in vitro Arabidopsis thaliana HPPD (AtHPPD) inhibitory activity and in vivo herbicidal activity were evaluated. The results of AtHPPD inhibitory experiments indicated that, most of the synthesized compounds showed "good" to "the excellent" HPPD-inhibiting activities. To our delight that, compound 9i with a Ki value of 0.005 μmol/L is about two times more potent than that of mesotrione (Ki=0.013 μmol/L). The results of greenhouse experiments showed that, most of the synthesized compounds displayed at least 80% inhibition against one of six weeds tested at the rate of 150 g ai/ha. To our surprise that, compound 9g showed over 85% inhibition against four of six tested weeds even at a rate as low as 37.5 g ai/ha. In addition, it was also safe for rice and wheat by post-emergent application at the rate of 150 g ai/ha. Furthermore, we also obtained some structure-activity relationships, the SAR indicated that too electron-withdrawing groups in 3,5-positions of benzene ring (3-position of quinazoline-2,4-dione) were detrimental to activity, too sterically bulk groups at R4 were also detrimental to activity. Thus, compound 9g emerged as a new lead compound for herbicidal discovery.

Key words: 4-hydroxyphenylpyruvate dioxygenase, quinazoline-2,4-dione, herbicidal activity, bioactivity study, lead com-pound