Acta Chim. Sinica ›› 2017, Vol. 75 ›› Issue (12): 1173-1182.DOI: 10.6023/A17090419 Previous Articles     Next Articles

Review

生物正交剪切反应及其应用

王杰a,b, 陈鹏a,b   

  1. a 北京大学化学与分子工程学院 北京 100871;
    b 北京大学前沿交叉学科研究院 北京 100871
  • 收稿日期:2017-09-14 出版日期:2017-12-15 发布日期:2017-10-09
  • 通讯作者: 陈鹏,E-mail:pengchen@pku.edu.cn E-mail:pengchen@pku.edu.cn
  • 作者简介:陈鹏,北京大学化学与分子工程学院教授、博士生导师,北大-清华生命科学联合中心高级研究员.2002年本科毕业于北京大学化学学院,2007年获得美国芝加哥大学博士学位,随后赴美国Scripps研究所进行博士后研究,2009年归国,任北京大学"百人计划研究员",2014年通过国际评估晋升长聘教授.科学研究集中在化学生物学领域,发展了"活细胞化学工具箱",实现了活体环境下的蛋白质特异标记、交联与操控,并开展了相应的生物学研究.2012年获得国家杰出青年科学基金,2015年成为基金委创新研究群体学术带头人,曾获陈嘉庚青年科学奖(2016)、教育部青年科学奖(2016)、国际生物无机化学会青年科学奖(SBIC award 2017)等荣誉;王杰,2012年本科毕业于武汉大学化学院,现为北京大学前沿交叉学科研究院博士研究生,导师陈鹏教授.主要研究方向是非天然氨基酸与蛋白质化学、生物正交断键反应的开发与应用.
  • 基金资助:

    项目受中国自然科学基金(Nos.21521003,21432002)资助.

Development and Applications of Bioorthogonal Cleavage Reactions

Wang Jiea,b, Chen Penga,b   

  1. a College of Chemistry and Molecular Engineering, Peking University, Beijing 100871;
    b Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871
  • Received:2017-09-14 Online:2017-12-15 Published:2017-10-09
  • Contact: 10.6023/A17090419 E-mail:pengchen@pku.edu.cn
  • Supported by:

    Project supported by the National Natural Science Foundation of China (Nos. 21521003, 21432002).

Bioorthogonal reactions enable us to study and manipulate biological processes under living conditions. As widely used and powerful tools, biorthogonal reactions are largely defined as "ligation reactions" that are used for labeling, tracing and capturing biomolecules. Recently, an emerging collection of biorthogonal "bond-cleavage reactions" have been developed and applied for biological studies, especially in releasing, activating and manipulating biomolecules. In this review, we will first summarize the characteristics and applications of these biorthogonal cleavage reactions. We will then focus on introducing diverse applications of biorthogonal cleavage reactions, including activation of prodrugs, rescue of intracellular protein activity, engineering of cell surface, among other interesting applications. Finally, the outlook of future development and applications of biorthogonal cleavage reactions will be discussed.

Key words: biorthogonal cleavage reaction, protein activation, pro-drug, unnatural amino acids, chemical decaging