Acta Chim. Sinica ›› 2018, Vol. 76 ›› Issue (5): 393-399.DOI: 10.6023/A18010039 Previous Articles     Next Articles



徐毅a,b, 赵彦b, 张叶俊c, 崔之芬b, 王丽华b, 樊春海b, 高基民a, 孙艳红b   

  1. a 温州医科大学 检验医学院、生命科学学院 温州 325000;
    b 中国科学院上海应用物理研究所 微观界面物理与探测重点实验室 物理生物学研究室 上海 201800;
    c 苏州影睿光学科技有限公司 苏州 215123
  • 投稿日期:2018-01-26 发布日期:2018-03-16
  • 通讯作者: 高基民,;孙艳红,;
  • 基金资助:


Angiopep-2-conjugated Ag2S Quantum Dot for NIR-II Imaging of Brain Tumors

Xu Yia,b, Zhao Yanb, Zhang Yejunc, Cui Zhifenb, Wang Lihuab, Fan Chunhaib, Gao Jimina, Sun Yanhongb   

  1. a School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325000;
    b CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800;
    c Suzhou Near-Infrared Optics Technology Co., Ltd., Suzhou 215123
  • Received:2018-01-26 Published:2018-03-16
  • Supported by:

    Project supported by the National Natural Science Foundation of China (Nos. 11575278, 21675167, 11675251, 61475181, 61378062), the Ministry of Science and Technology of China (Nos. 2016YFA0201200, 2016YFA0400902) and the Key Research Program of Frontier Sciences (No. QYZDJ-SSW-SLH031-02).

Ag2S quantum dot with excellent NIR-Ⅱ fluorescence can provide deeper tissue penetration (>1.1 cm) and higher spatiotemporal resolution (25 μm, 50 ms) in comparison to the conventional fluorophore. In this study, we designed a NIR-Ⅱ probe based Ag2S quantum dot for imaging of brain tumor. Angiopep-2 was used to modify Ag2S quantum dot, which is a 19-mer peptide exhibiting high binding efficiency with low-density lipoprotein receptor-related protein-1 (LRP-1) of blood brain barrier and glioma. Due to the surface of Ag2S quantum dots with carboxyl groups and angiopep-2 peptide with amino groups, Ag2S was conjugated with Angiopep-2 (Ag2S-ANG) through the condensation reaction of amino and carboxyl groups mediated by EDC and NHS. The structure, size and spectral properties of Ag2S-ANG were characterized by agarose electrophoresis, dynamic light scattering transmission, electron microscope (TEM), UV-vis spectrometer and NIR fluorescence spectrometer, respectively. Results showed that Ag2S-ANG had a short migration distance compared with Ag2S in the agarose gel electrophoresis. The hydrate particle size of Ag2S was approximately 6 nm, Ag2S-ANG was approximately 8 nm and its zeta potential exhibited electropositive reinforcement, zeta potential of Ag2S is -11.47±1.56 mV and Ag2S-ANG is +28.7±1.35 mV. Ag2S-ANG exhibited similar absorbance and fluorescence spectra to Ag2S, except a slight enhancement of emission peak. These results indicated that Ag2S-ANG was synthesized successfully. We further observed its cell cytotoxicity, distribution and uptake in Uppsala 87 Malignant Glioma cells(U87MG), and in vivo distribution in the solid tumor-bearing mouse. Ag2S-ANG had no obvious cytotoxicity when the concentration is inferior to 100 μg/mL and had more uptake in U87MG cells than that of Ag2S. In animal experiments, glioma tumor-bearing mice were used to investigate the distribution and tumor targeting of Ag2S-ANG. Results showed that Ag2S-ANG can distribute and accumulate in subcutaneous tumor site, indicating that Ag2S-ANG had the potential of targeting the glioma cells.

Key words: Ag2S quantum dot, brain-targeting, angiopep-2, NIR-II imaging References