In a previous paper^[1] of this series of investigations the syntheses of p-,m-,and o-(bis-(β-chloroethyl)-amino]-methyl-phenylalanine dihydrochlorides (Ⅱ, designated as AT-290, AT-582, and AT-581 respectively) by substituting one hydrogen atom of the methyl group of HN₂ with the phenylalanyl group, had been described. In this investigation, 2-and 3-[bis-β-chloroethyl)-amino]-methyl-4-hydroxyphenylalanine dihydrochlorides (Ⅳ and Ⅴ, designated as AT-786 and AT-916 respectively) had been synthesized, however, by substituting one hydrogen atom of the methyl group of HN₂ with the tyrosinyl group.