Acta Chimica Sinica ›› 1981, Vol. 39 ›› Issue (6): 553-561. Previous Articles     Next Articles

核酸化学研究Ⅳ.常见核糖核苷及其3’-核糖核苷酸柱层析

核酸组   

  1. 中国科学院上海有机化学研究所
  • 收稿日期:1979-11-02 出版日期:1981-11-15 发布日期:2013-06-03

STUDIES ON NUCLEIC ACID CHEMISTRY Ⅳ. THE COLUMN CHROMATOGRAPHY OF COMMON RIBONUCLEOSIDES AND RIBONUCLEOTIDES

NUCLEIC ACID GROUP   

  1. Shanghai Institute of Organic Chemistry, Academia Sinica
  • Received:1979-11-02 Online:1981-11-15 Published:2013-06-03

A simple method for the separation and quantitative analysis of the font common ribonuoleosides and their 3'-ribonuoleotides by reversed phase anion-ezohange chromatography is described.A column of polyohlorotrifluoroethylene(16O-200 mesh) coated with 12%(ml/g)methyl-tri-n-alkyl(C8-C10) ammonium chloride and a stepwise elution process are adopted.The volnmns and concentrations of aqueous ymmoninm carbonate eluent(pH 8.2) employed in succession are 30 ml of 0.001 M,200 ml of 0.2 M and 10 ml of 0.3 M.