Acta Chimica Sinica ›› 1994, Vol. 52 ›› Issue (5): 496-499. Previous Articles     Next Articles

Original Articles

色氨酸-亚硝酸体系荧光性质的研究及应用

揭念琴;杨景和;孟凡琴;黄慕宾   

  1. 山东大学化学系.济南(250100)
  • 出版日期:1994-05-15 发布日期:1994-05-15

Fluorescence properties of tryptophan-nitrous acid system and its application

Jie Nianqin;Yang Jinghe;Meng Fanqin;Huang Mubin   

  1. Shandong Univ, Dept Chem.Jian(250100)
  • Online:1994-05-15 Published:1994-05-15

Tryptophan is oxidized by nitrous acid at elevated temperature produce a phenolic intermediate which further reacts with nitrous acid to form a fluorescent species. The flluorescent species has the excitation and emission maxima at 320 and 392nm, respectively. The fluorescent intensity is linear over the range of 0.2-1.6μg/mL tryptophan. The method has been applied to the determination of tryptophan in synthetic and protein samples. A plausible mechanism is proposed to explain this behaviour.

Key words: FLUORESCENCE CHARACTERISTIC, NITROUS ACID, TRYPTOPHAN, PROTEIN, FLUOROSPECTROPHOTOMETRY

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