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Acta Chimica Sinica ›› 1999, Vol. 57 ›› Issue (10): 1142-1146. Previous Articles Next Articles
Original Articles
黎军;邓慧敏;赖志辉;赵善楷
发布日期:
Li Jun;Deng Huimin;Lai Zhihui;Zhao Shankai
Published:
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This paper reports the study on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) of DNA, using 2, 4, 6-trihydroxyacetophenone (2, 4, 6-THAP) as matrix. The results show that 2, 4, 6-THAP has high efficiency in desorbing and ionizing DNA molecules: for [M-H]^- of d(T)~1~0, the resolution is 1130.0, the signal-to-noise ratio is 366.0, and the detection limit is 5×10^-^1^4mol; it can measure mix-based DNA with molecular weight higher than 14800. In order to improve desorption/ionization effects, the purification methods for DNA samples are established.
Key words: ACETOPHENONE P, NUCLEIC ACIDS, LASER, DESORPTION, TIME-OFF FLIGHT MASS SPECTROMETRY
CLC Number:
O657
Li Jun;Deng Huimin;Lai Zhihui;Zhao Shankai. A study of using 2, 4, 6-trihydroxyacetophenone as matrix in matrix- assisted laser desorption/ionization mass spectrometry for DNA detection[J]. Acta Chimica Sinica, 1999, 57(10): 1142-1146.
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