Acta Chimica Sinica ›› 1999, Vol. 57 ›› Issue (6): 572-577. Previous Articles     Next Articles

Original Articles

荧光法研究抗癌药物更生霉素D与小牛胸腺DNA的作用机理

李来生;黄伟东;王瑞琼;鄢远   

  1. 南昌大学应用化学研究所
  • 出版日期:1999-06-15 发布日期:1999-06-15

Study on the mechanism of the interaction between anticancer drug actinomycin D and calf thymus DNA by fluorescence method

Li Laisheng;Huang Weidong;Wang Ruiqiong;Yan Yuan   

  • Online:1999-06-15 Published:1999-06-15

The mechanism of the interaction between actionmycin D(ACTD) and calf thymus (CT) DNA has been studied by fluorescence method using ethidium bromide (EB) as a fluorescence probe. The results from varied experiments such as fluorescence spectra, fluorescence polarization, Scatchard curve, DNA heat denaturation, and DNA salt effect etc., support that intercalation and groove binding are the two major modes for the interaction ACTD and CT DNA. The absence of the electrostatic binding between the ACTD and the phosphate backbone of CT DNA has been proved.

Key words: ANTICARCINOGEN, DEOXYRIBONUCLEIC ACID, ETHIDIUM BROMIDE, FLUOROSPECTROPHOTOMETRY

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