Acta Chimica Sinica ›› 2001, Vol. 59 ›› Issue (5): 742-745. Previous Articles     Next Articles

Original Articles

Ce^4+水解切籽5'-AMP,3'-AMPA和CAMP的原位增强 拉曼光谱研究

沈鹤柏;夏静芬;杨海峰;蒯丽蘅   

  1. 上海池大学生命与环境科学学院
  • 出版日期:2001-05-15 发布日期:2001-05-15

Study on in situ surface enhanced raman scattering (SERS) of 5'-AMP, 3'-AMP and CAMP hydrolysis by Ce^4+ ion

Shen Hebai;Xia Jingfen;Yang Haifeng;Kuai Liheng   

  • Online:2001-05-15 Published:2001-05-15

Surface enhanced Raman scattering (SERS) spectra of nucleotide hydrolysis mediated by Ce^4+ ion are reported. The experimental results demonstrated that excellent SERS spectra of 5'---AMP,3'-AMP and CAMP could be obtained on rough silver surface. New additional peaks at 2 895cm^-1 and 2 950cm^-1 were observed after5'---AMP hydrolysis by Ce^4+. These signals were all assigned to the vibration of CH~2. These signals were also observed with 3'-AMP but thry were either weakentened or disappeared after hydrolysis of the 3'-AMP by Ce^4+. comparing the in situ SERS spectra of CAMP hydrolysis by Ce^4+ ion with those of 5'---AMP,3'-AMP reaction with Ce^4+ ion, we conclude that in the begining Ce^4+ ion only cleaves one phosphate ester, giving a mixture of 5'---AMP and 3'-AMP, with the reaction processed, another phosphate ester is also hydrilysis to yield adenosine.

Key words: CERIUM, HYDROLYSIS, NUCLEOTIDES

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