Acta Chimica Sinica ›› 2002, Vol. 60 ›› Issue (6): 967-972. Previous Articles     Next Articles

Original Articles

巯嘌呤金属配合物与小牛胸腺DNA的作用

卢继新;张贵珠;黄志娜;赵鹏   

  1. 天津医科大学药学院.天津(300203);南开大学化学系.天津(300071)
  • 出版日期:2002-06-15 发布日期:2002-06-15

Study on the Mechanism of the Interaction between Mercaptopurine Metal Complexes and Calf Thymus DNA

Lu Jixin;Zhang Guizhu;Huang Zhina;Zhao Peng   

  1. Department of Pharmacy, Tianjin Medical University.Tianjin(300203);Department of Chemistry, Nankai University.Tianjin(300071)
  • Online:2002-06-15 Published:2002-06-15

The mechanism of the interaction between mercaptopurine (MP) metal (Me) complexes and calf thymus (CT) DNA has been studied by using ethidium bromide (EB) as a fluorescence probe. The results from varied experiments such as ultraviolet and fluorescence spectra, fluorescence quenching curve and Scatchard equation and circular dichroism, etc., confirm that intercalation and electrostatic binding are the two major modes for interaction between MP-Me complexs and CT DNA. The absence of the intercalation binding between the MP and CT DNA has been proved. The binding constant of MP-Me complexs with CT DNA has been determined.

Key words: MERCAPTOPURINE, ethidium bromide, CALF, THYMUS GLAND, COMPLEX COMPOUNDS

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