Acta Chimica Sinica ›› 2005, Vol. 63 ›› Issue (23): 2153-2157. Previous Articles     Next Articles

全反式维甲酸合铜(II)配合物对DNA的切割和键合作用

杨培菊1,2, 孙坤3, 苏雪3, 王流芳4, 宋玉民1   

  1. 1. 西北师范大学化学化工学院, 兰州, 730070;
    2.中国科学院兰州化学物理研究所, 羰基合成与选择氧化国家重点实验室, 兰州, 730000;
    3. 西北师范大学生命科学学院, 兰州, 730070;
    4. 兰州大学功能有机分子化学国家重点实验室, 兰州, 730000
  • 投稿日期:2004-12-01 修回日期:2005-06-07 发布日期:2014-02-14
  • 通讯作者: songym@nwnu.edu.cn
  • 作者简介:宋玉民,E-mail:songym@nwnu.edu.cn
  • 基金资助:

    国家自然科学基金(No.30270091);甘肃省自然科学基金(No.3zs051-Az5-021);甘肃省高分子材料重点实验室西北师范大学A级重点学科资助项目.

Study on the Interactions and Cleavage of Cu(RA)2·3H2O with DNA

YANG Pei-Jua1,2, SUN Kun3, SU Xue3, WANG Liu-Fang4, SONG Yu-Min1   

  1. 1. College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070;
    2. State Key Laboratory of Carbonyl Synthesis and Oxidation, Institute of Chemistry and Physics, Chinese Academy of Sciences, Lanzhou 730000;
    3. College of Life Science, Northwest Normal University, Lanzhou 730070;
    4. State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000
  • Received:2004-12-01 Revised:2005-06-07 Published:2014-02-14

The interactions of the Cu(II) complex of all-trans retinoic acid [Cu(RA)2·3H2O] with DNA have been studied using agarose gel electrophoresis, fluorescence spectroscopy, viscosity measurement and electrochemical methods. The results indicate that the Cu(RA)2·3H2O can effectively promote the cleavage of plasmid DNA at physiological pH and temperature, and also increase the viscosity of DNA. The emission intensity was quenched as Cu(RA)2·3H2O was added to the EB-DNA system. The results above suggest that the complex should bind DNA mainly by intercalation, to cause a decrease of anodic peak current in their cyclic voltammograms and exhibit enhanced DNA binding.

Key words: Cu(RA)2·3H2O, calf thymus DNA, cleavage, electrochemistry