Acta Chimica Sinica ›› 2007, Vol. 65 ›› Issue (7): 640-644. Previous Articles     Next Articles

Original Articles

亮氨酸与异亮氨酸的表面增强拉曼光谱

周光明*, 虞丹尼, 黎司, 杨大成   

  1. (西南大学化学化工学院 重庆 400715)
  • 收稿日期:2006-08-05 修回日期:2006-09-21 出版日期:2007-04-14 发布日期:2007-04-14
  • 通讯作者: 周光明

Surface Enhanced Raman Spectroscopy of Leucine and Isoleucine

ZHOU Guang-Ming*; YU Dan-Ni; LI Si; YANG Da-Cheng   

  1. (School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715)
  • Received:2006-08-05 Revised:2006-09-21 Online:2007-04-14 Published:2007-04-14
  • Contact: ZHOU Guang-Ming

FT-Raman and surface enhanced Raman spectra (SERS) of leucine and isoleucine, the only isomer in proteinic amino acids, on the silver colloidal substrate are recorded. The vibrational and enhanced peaks are assigned; the Raman shifts that stem from different vibrational model in the molecular inner structure, and the variations of SERS at different pH values are analyzed. One methyl’s different connection to the main chains of the isomer amino acid resulted in different vibrational model in inner molecule, expressed in Raman spectra: ρ(CH3) and δas(CH3) of leucine are at 962, 945, 924 and 1454, 1408 cm-1, by the comparation, isoleucine’s are at 922 and 1448, 1420, 1394 cm-1. Vibrations involved in C—CO, C—C, H—O…H and lattice vibration mode are identical. In saturated solution’s Raman spectra and SERS, all the different shifts are expressed more clearly than those in solid state’s Raman spectra. The adsorption models of the isomer amino acid on the silver surface are speculated.

Key words: leucine, isoleucine, FT-Raman, FT-SERS, pH value