Acta Chimica Sinica ›› 2008, Vol. 66 ›› Issue (12): 1455-1459. Previous Articles     Next Articles

基于金属离子螯合的压电免疫传感器新型固定化方法

陈 媛 吴朝阳* 沈国励 俞汝勤   

  1. (湖南大学化学化工学院 化学生物传感与计量学国家重点实验室 长沙 410082)
  • 收稿日期:2007-11-19 修回日期:2008-03-21 出版日期:2008-06-28 发布日期:2008-06-28
  • 通讯作者: 吴朝阳

A New Metal Ion Chelation Based Immobilization Approach for Piezoelectric Immunosensors

CHEN, Yuan WU, Zhao-Yang* SHEN, Guo-Li YU, Ru-Qin   

  1. (State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering,
    Hunan University, Changsha 410082)
  • Received:2007-11-19 Revised:2008-03-21 Online:2008-06-28 Published:2008-06-28
  • Contact: WU, Zhao-Yang

A new antibody immobilization approach has been proposed for the piezoelectric immunosensors through metal ion chelation. The piezoelectric quartz crystal surfaces were first coated with the plasma-polymerized films of butyl amine (BA-PPF) by using a radio frequency plasma deposition technique. The BA-PPFs were modified with amine triacetic acid groups, and the diethylenetriaminepentaacetic acid-modified IgG antibody (DTPA-IgG Ab) was then immobilized through mediating chelation of metal ions. The piezoelectric immunosensor could detect the normal human IgG in the range of 0.36~63.8 mg/mL. This simple and effective immobilization approach is of certain potential to be a common immobilization for piezoelectric immunosensor construction.

Key words: plasma-polymerized film, metal ion chelation, immobilization approach