Acta Chimica Sinica ›› 2008, Vol. 66 ›› Issue (5): 525-530. Previous Articles     Next Articles

Original Articles

16-O-去甲基乌头碱的生物转化及电喷雾质谱研究

赵宇峰1,2,宋凤瑞1,王曦烨1,2,国新华1,刘志强1,刘淑莹*,1   

  1. (1中国科学院长春应用化学研究所 长春 130022)
    (2中国科学院研究生院 北京 100039)
  • 收稿日期:2007-04-03 修回日期:2007-10-29 出版日期:2008-03-14 发布日期:2008-03-14
  • 通讯作者: 刘淑莹

Studies on the Biotransformation of 16-O-Demethylaconitine and Electrospray Ionization Tandem Mass Spectrometry

ZHAO Yu-Feng1,2 SONG Feng-Rui1 WANG Xi-Ye1,2 GUO Xin-Hua1, LIU Zhi-Qiang1, LIU Shu-Ying*,1   

  1. (1 Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022)
    (2 Graduate School of the Chinese Academy of Sciences, Beijing 100039)
  • Received:2007-04-03 Revised:2007-10-29 Online:2008-03-14 Published:2008-03-14
  • Contact: LIU Shu-Ying

To study the biotransformation of 16-O-demethylaconitine in human intestine, aconitine was incubated with human intestinal bacteria in vitro. The metabolites of 16-O-demethylaconitine were investigated using ion trap and Fourier transform ion cyclotron resonance electrospray ionization tandem mass spectrometry directly. Aconitum alkaloids formed protonated molecule [M+H] in the positive ion mode. It was found that 16-O-demethylaconitine from biotransformation of aconitine could be further transformed by human intestinal bacteria. There are new metabolites such as mono-ester alkaloids, diester alkaloids and lipo-alkaloids produced by deacetylation, debenzoylation, dehydroxylation, demethylation and more than twenty kinds of esterification in the further transformation process of the 16-O-demethylaconitine. The 16-O- lipodemethylaconitines have been first reported as a metabolite of aconitine by human intestinal bacteria.

Key words: 16-O-demethylaconitine, human intestinal bacteria, biotransformation, electrospray ionization mass spectrometry