Acta Chimica Sinica ›› 2008, Vol. 66 ›› Issue (5): 581-586. Previous Articles     Next Articles

Original Articles

水溶性有机物与细胞膜的结合作用

李璐,吴玲玲,郜洪文*,潘琦,李硕   

  1. (同济大学环境科学与工程学院 污染控制与资源化研究国家重点实验室 上海 200092)
  • 收稿日期:2007-06-07 修回日期:2007-08-27 出版日期:2008-03-14 发布日期:2008-03-14
  • 通讯作者: 郜洪文

Binding of Water-soluble Organic Compounds to Cellular Membrane

LI Lu, WU Ling-Ling, GAO Hong-Wen*, PAN Qi LI Shuo   

  1. (State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092)
  • Received:2007-06-07 Revised:2007-08-27 Online:2008-03-14 Published:2008-03-14
  • Contact: GAO Hong-Wen

The binding of ionic organic compounds, anionic diamine blue 3R (DAB) and cationic rhodamine B (RB) to in vitro single phospholipids membrane (SPM) and Escherichia coli (E. coli) strains was investigated. The interactions correspond to the Langmuir and Temkin adsorption isotherm in only monolayers. The influence of ionic strength, pH and temperature on the adsorption were examined and analyzed. The binding force, type and interaction sites of DAB and RB were determined and identified. The interaction of DAB and RB with SPM and the cells are exothermal and idiopathic, which contained the union of non-covalent bonds, such as electrostatic bond, hydrogen bond and van der Waals force. Moreover, most of DAB and RB were accumulated on the excellular membrane and walls, to hardly enter the cytoplasm. Besides, the adsorption competition was found when both DAB and RB existed simultaneously.

Key words: ionic organic compound, single phospholipid membrane, cellular membrane, E. coli, chemical adsorption