Acta Chimica Sinica ›› 2010, Vol. 68 ›› Issue (17): 1758-1764. Previous Articles     Next Articles

Full Papers

硫酸氧钒毒性的核磁共振代谢组学方法研究

董继扬1,危阳洋1,2,戴晓侠2,邓伶莉1,陈忠*,1,2   

  1. (1厦门大学物理系 固体表面物理化学国家重点实验室 厦门 361005)
    (2厦门大学生物材料系 厦门 361005)
  • 收稿日期:2009-10-21 修回日期:2010-03-02 出版日期:2010-09-14 发布日期:2010-04-18
  • 通讯作者: 董继扬 E-mail:jydong@xmu.edu.cn
  • 基金资助:

    卫生部科学研究基金—福建省卫生教育联合攻关计划(WKJ2008-2-36);福建省自然科学基金(2009J01299)

Toxicity Study of VOSO4 Using NMR-based Metabonomics

Dong Jiyang1 Wei Yangyang1,2 Dai Xiaoxia2 Deng Lingli1 Chen Zhong*,1,2   

  1. (1 State Key Laboratory of Physical Chemistry of Solid Surface, Department of Physics, Xiamen University, Xiamen 361005)
    (2 Department of Biomaterials, Xiamen University, Xiamen 361005)
  • Received:2009-10-21 Revised:2010-03-02 Online:2010-09-14 Published:2010-04-18
  • Contact: Ji-Yang Dong E-mail:jydong@xmu.edu.cn

NMR-Based metabonomics combined with clinical biochemical analysis and histopathological examination was applied to investigate the toxicity effects of vanadyl sulfate with insulin-like activity in male Wistar rats. Male Wistar rats were administrated with VOSO4 at doses of 15 and 45 mg/kg body weight by intragastric administration for 16 d. Urine and serum samples were collected and analyzed by 1H NMR experiment. Multivariate analyses were employed to identify the characteristic metabolites for the toxicity effects of vanadyl sulfate. Then the metabolic networks of these characteristic metabolites were built up using TICL (a web Tool for automatic Interpretation of Compound List). The relationship between the characteristic metabolites and the main matebolic pathways perturbed were analyzed and discussed. The differences of metabolic profiles were examined among high-dose (45 mg/kg), low-dose (15 mg/kg) of VOSO4 and control groups. Compared to the control group, increased levels of lactate, creatinine and taurine in serum and increased excretion of trymethylamine-N2-oxide, creatinine, taurine and glycine in urine were found in both high- and low-dose groups which showed an obvious dose-dependent relationship. On the other hand, the concentration of acetate and succinate were decreased in both serum and urine samples of dosed groups. These results indicate that VOSO4 have disturbed the carbohydrate metabolism, lipid metabolism and gut microflora and the high-dosage of VOSO4 may cause liver and kidney injury.

Key words: nuclear magnetic resonance (NMR), metabonomics, toxicity, vanadyl sulfate, a web tool for automatic interpretation of compound list (TICL)