Acta Chimica Sinica ›› 2011, Vol. 69 ›› Issue (19): 2265-2271. Previous Articles     Next Articles

Full Papers

基于1H NMR代谢组学方法的糖尿病溃疡组织代谢特征和病理机制分析


  1. (1温州医学院药学院 温州 325035)
    (2上海交通大学医学院附属仁济医院泌尿科 上海 200127)
  • 投稿日期:2010-11-28 修回日期:2011-06-02 发布日期:2011-06-21
  • 通讯作者: 谢丽云
  • 基金资助:


Studies on Metabolic Characteristics and Pathological Mechanisms of Diabetic Ulcers by 1H NMR-based Metabonomics

Xie Liyun1 Song Caiyong1 Lou Yiyi 1 Xu Zhi 1 Zhang Meiling1 Dong Baijun2 Gao Hongchang*,1 Li Xiaokun1   

  1. (1 School of Pharmacy, Wenzhou Medical College, Wenzhou 325035)
    (2 School of Medicine, Shanghai Jiao Tong University, Shanghai 200127)
  • Received:2010-11-28 Revised:2011-06-02 Published:2011-06-21

Diabetic ulcer is the most important factor leading to clinical amputation. In the present study, 1H NMR-based metabonomics combined with pathological and biochemical analysis were used to study the metabolic characteristics and pathological mechanisms of diabetic ulcers. The results showed that the fibroblast and blood capillaries proliferation in skin ulcers of diabetic rats were significantly weaker than those in the control rats. From the scores plots of metabonomic studies, we found distinct, easily detectable differences between (a) diabetic ulcer rats and control rats, and (b) diabetic ulcer rats with different healing time. Compared to control skin ulcers, diabetic skin ulcers had lower levels of leucine, isoleucine, alanine, citrate, choline and betaine, together with higher levels of lactate, acetate, pyruvate, creatine, taurine and glycine. Altered metabolite concentrations are most likely the result of the cells switching to glycolysis and creatine metabolism to maintain energy homeostasis following the loss of ATP caused by impaired TCA cycle in diabetic ulcers and to repair the skin trauma. The present study may be able to assist deeply understanding of diabetic ulcers and selection of treatment protocols.

Key words: diabetic ulcer, 1H NMR, pathological mechanisms, skin tissue

CLC Number: