Acta Chimica Sinica ›› 2009, Vol. 67 ›› Issue (18): 2116-2120. Previous Articles     Next Articles

Original Articles

微乳液相色谱测定药物的油-水分配系数的新方法研究

李 宁 黄纪云 黄碎锦 高崇凯*   

  1. (广东药学院 广州 510006)
  • 收稿日期:2009-01-20 修回日期:2009-03-16 出版日期:2009-09-28 发布日期:2009-12-31
  • 通讯作者: 高崇凯

A Novel Determination of Oil-Water Partition Coefficients for Pharmaceuticals by Microemulsion Liquid Chromatography

Li, Ning Huang, Jiyun Huang, Suijin Gao, Chongkai*   

  1. (Guangdong College of Pharmacy, Guangzhou 510006)
  • Received:2009-01-20 Revised:2009-03-16 Online:2009-09-28 Published:2009-12-31
  • Contact: Gao, Chongkai

A rapid screening assay determination of octanol-water partition coefficients (log P) of pharmaceuticals was developed by using microemulsion liquid chromatography (MELC). Seven standard compounds with known log P values were used for constructing the calibration curve of log P against the MELC log k, by which the log P values of the pharmaceuticals were calculated. The types of surfactants and oil phase were evaluated in the microemulsion systems using such parameters as linear correlation coefficients the literature log P values against the experimentally measured log k values. The optimized MELC system with microemulsion consisting of 6.0% polyoxyethylene lauryl ether (Brij35)-6.6% butanol-0.8% octanol-86.6% phosphate buffer (0.05 mol•L-1, pH 7.0) shows good correlation (r=0.96) to the experimental log P (Elog P) values for noncongeneric neutral and basic pharmaceuticals. The average error of log P values measured by MELC is within 0.3 log unit of the values from the literature. The results indicate that MELC can serve as a high throughput, reliable and robust approach and as a valid model for log P determinations.

Key words: microemulsion liquid chromatography, oil-water partition coefficient, log P