Study on the Interaction between Riboflavin and Herring Sperm DNA by Fluorescence Spectrometry

Acta Chimica Sinica ›› 2008, Vol. 66 ›› Issue (10): 1245-1251. Previous Articles Next Articles

Original Articles

荧光光谱法研究核黄素与鲱鱼精DNA的相互作用

胡亚敏¹，王兴明*^,1，费丹¹，董发勤¹，丁立生²

(¹西南科技大学材料科学与工程学院化学系绵阳 621010)
(²中国科学院成都生物研究所成都 610041)

投稿日期:2007-08-06 修回日期:2007-12-09 发布日期:2008-05-28
通讯作者: 王兴明

Study on the Interaction between Riboflavin and Herring Sperm DNA by Fluorescence Spectrometry

HU, Ya-Min¹, WANG, Xing-Ming*^,1, FEI, Dan¹, DONG, Fa-Qin¹, DING, Li-Sheng²

(¹ Department of Chemistry, Southwest University of Science and Technology, Mianyang 621010)
(² Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041)

Received:2007-08-06 Revised:2007-12-09 Published:2008-05-28
Contact: WANG, Xing-Ming

Abstract

The interaction of Riboflavin with DNA was studied in a Britton-Robinson buffer solution by employing acridine orange as a fluorescence probe. The fluorescence spectra indicated that a kind of compound of Riboflavin and DNA was formed when pH was 7.25. The binary compound ratio is n_RF∶n_DNA＝5∶1; the binding constant of Riboflavin with DNA compound is 2.53×10⁶ L/mol at a temperature of 28 ℃; and the thermodynamic parameters of the interaction by a double reciprocal method were calculated: ＝－6.06×10⁴ J/mol, ＝－78.70 J/(mol•K), ＝－3.69×10⁴ J/mol at 28 ℃. The interaction of Riboflavin with DNA was studied also through methods of viscosity and Scatchard as well as salt effect. The results confirm that the groove binding and electrostatic binding are the two major modes of the interaction between Riboflavin and herring sperm DNA.

Key words: Riboflavin, Acridine orange, herring sperm DNA, action mode

Cite this article

HU, Ya-Min, WANG, Xing-Ming*^,1, FEI, Dan, DONG, Fa-Qin, DING, Li-Sheng². Study on the Interaction between Riboflavin and Herring Sperm DNA by Fluorescence Spectrometry[J]. Acta Chimica Sinica, 2008, 66(10): 1245-1251.

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荧光光谱法研究核黄素与鲱鱼精DNA的相互作用

Study on the Interaction between Riboflavin and Herring Sperm DNA by Fluorescence Spectrometry

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