A chiral stationary phase (CSP) was prepared by coating cellulose- tris (3,5-dimethylphenylcarbamate) onto aminopropylated spherical silica gel. The enantioselective separation of a series of biphenyl compounds with antihepatitis activity was achieved for the first time on the CSP, using the normalphase mode. The effects of temperature and concentration of ethanol in the mobile phase on the chiral separation were studied. the retention factors and separation factors for the enantiomers of all investigated compounds decreased with increasing temperature or increasing ethanol concentration in the eluent. The natural logarithms of the separation factors (lnα) of the investigated compounds depended linearly on the reciprocal of temperature (1/T). The thermodynamic parameters, such as the difference in enthalpy △(△H°), entropy △(△S°) and Gibbs free energy △(△G°) for the transfer of analyte from the mobile phase to the CSP were calculated.

Key words: DIPHENYL P, CELLULOSE, STATIONARY PHASE, HIGH SPEED LIQUID CHROMATOGRAPHY