Acta Chimica Sinica ›› 2005, Vol. 63 ›› Issue (12): 1147-1150. Previous Articles     Next Articles

Reports

膜片钳法研究镁离子对萝卜液泡膜SV通道的影响

张朝峰,杨频*,薛绍武   

  1. (山西大学分子科学研究所 化学生物学与分子工程教育部重点实验室 太原 030006)
  • 收稿日期:2004-12-22 修回日期:2005-02-28 出版日期:2005-06-28 发布日期:2010-12-10
  • 通讯作者: 杨频

Study of Magnesium Effects on Slow Vacuolar Ion Channel in Radish by Patch-Clamp Technique

ZHANG Chao-Feng, YANG Pin*, XUE Shao-Wu   

  1. (Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006)
  • Received:2004-12-22 Revised:2005-02-28 Online:2005-06-28 Published:2010-12-10
  • Contact: YANG Pin

The effects of magnesium cation on slow vacuolar (SV) channel currents in radish were studied using the whole-vacuole patch-clamp technique. In the presence of Ca2+ buffer EGTA, cytosolic Mg2+could not activate SV currents instead of cytosolic Ca2+. Mg2+added to bath solution caused a decrease of SV currents in a concentration-dependent manner. The concentration response curve could be fitted to a Hill equation with a Ki of (1.94±0.11) mmol/L. Varying the luminal Mg2+concentration had no effect on SV currents. These results will provide an important basis at channel level for the further research of the effects of magnesium on physiological activities of plants.

Key words: SV channel, cytosolic Mg2+, patch-clamp, vacuole