Acta Chimica Sinica ›› 2011, Vol. 69 ›› Issue (16): 1951-1956. Previous Articles    

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碲化镉量子点作为探针研究核黄素与鲑鱼精DNA的相互作用

张渝阳*,1,3,李荧荧1,赵琨2,3,赵玲1,臧树良1,3   

  1. (1辽宁大学化学院 沈阳 110036)
    (2沈阳药科大学药学院 沈阳 110016 )
    (3辽宁大学稀散元素研究所 沈阳 110036)
  • 投稿日期:2010-12-03 修回日期:2011-04-01 发布日期:2011-04-20
  • 通讯作者: 张渝阳 E-mail:z6531098@163.com
  • 基金资助:

    国家自然科学基金

CdTe Quantum Dots as a Probe for Interaction between Salmon Sperm DNA and Riboflavin

Zhang Yuyang*,1,3 Li Yingying1 Zhao Kun2,3 Zhao Ling1 Zang Shuliang1,3   

  1. (1 College of Chemistry, Liaoning University, Shenyang 110036)
    (2 School of Pharmacy, Shengyang Pharmaceutical University, Shenyang 110016)
    (3 Institute of Rare and Scattered Elements, Liaoning University, Shenyang 110036)
  • Received:2010-12-03 Revised:2011-04-01 Published:2011-04-20
  • Contact: Zhang YuYang E-mail:z6531098@163.com

The water-soluble CdTe quantum dots (QDs) were synthesized using mercaptopropionic acid as stabilizer. The interaction of riboflavin (RF) with salmon sperm DNA was investigated using UV and fluorescent spectra by employing CdTe QDs as a fluorescence probe in pH 7.25 Britton-Robinson (B-R) buffer solution. Decreased fluorescence and hypochromism of UV spectra were observed as RF interaction with DNA. The interaction of riboflavin with DNA has also been studied through methods of salt effect and thermal denaturation experiment. The experimental results confirm that the action mode of RF to DNA is electrostatic binding and intercalation.

Key words: CdTe quantum dots, riboflavin, salmon sperm DNA, fluorescent spectrum, intercalation