Acta Chimica Sinica ›› 2011, Vol. 69 ›› Issue (23): 2877-2881.DOI: 10.6023/A1106062 Previous Articles     Next Articles

Full Papers

在硅胶表面制备牛血红蛋白分子印迹聚合物

郭敏杰*, 宋艾芳, 樊志, 么敬霞   

  1. (天津科技大学理学院化学系 天津 300457)
  • 收稿日期:2011-06-06 修回日期:2011-08-01 出版日期:2011-12-14 发布日期:2011-08-15
  • 通讯作者: 郭敏杰 E-mail:guomj@tust.edu.cn
  • 基金资助:

    国家自然科学基金;天津市自然科学基金

Imprinting Recognition of Bovine Hemoglobin with Assistant Recognition Polymer Chains on Silica Surface

GUO Min-Jie, SONG Ai-Fang, FAN Zhi, ME Jing-Xia   

  1. (Department of Chemistry, College of Sciences, Tianjin University of Science and Technology, Tianjin 300457)
  • Received:2011-06-06 Revised:2011-08-01 Online:2011-12-14 Published:2011-08-15
  • Contact: Minjie Guo E-mail:guomj@tust.edu.cn

A new protein imprinted polymer was prepared on silica surface modified with vinyltrimetho xysilane, using acrylamide as the functional monomer, N,N-methylene bisacrylamide as the cross linker and modified polyvinyl alcohol (PVA) as the assistant recognition polymer chains (ARPCs). The framework of ARPCs was analyzed by the infrared spectrum (IR). Scanning electron microscopy (SEM) images demonstrated the different morphologies. The effect of ARPCs contents on the MIP absorption ability was investigated. The study of adsorption kinetics showed that the adsorption capacity of molecularly imprinted polymers (MIPs) was obviously improved when ARPCs were introduced into the polymer matrix. Competitive adsorption experiments were used to investigate the selectivity to the template bovine hemoglobin (BHb) from the mixture of other proteins. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) results showed that MIPs had excellent specific adsorption ability to the template BHb.

Key words: surface imprinting, assistant recognition polymer chain, polyvinyl alcohol, silica bead