Acta Chimica Sinica ›› 2012, Vol. 70 ›› Issue (11): 1263-1270.DOI: 10.6023/A1109192 Previous Articles     Next Articles

Full Papers


李卫兵, 乔赫元, 赵刚   

  1. 中国科学技术大学 电子科学与技术系 低温生物医学工程实验室, 安徽合肥 230027
  • 投稿日期:2011-09-19 修回日期:2012-03-30 发布日期:2012-03-30
  • 通讯作者: 赵刚
  • 基金资助:

    国家自然科学基金(No. 51076149)和中央高校基础科研业务费专项基金资助.

Effect of Non-ideality of Intracellular Solution on the Optimal Protocols for Cell Cryopreservation

LI Wei-Bing, QIAO He-Yuan, ZHAO Gang   

  1. University of Science and Technology of China, Department of Electronic Science and Technology, Laboratory of Cryo-Biomedical Engineering, Hefei 230027, Anhui Province
  • Received:2011-09-19 Revised:2012-03-30 Published:2012-03-30
  • Supported by:

    Supported by the National Natural Science Foundation of China (No. 51076149) and the Fundamental Research Funds for the Central Universities.

To predict the optimal cryopreservation protocol for biological cells, a new intracellular ice formation model was developed by incorporating the nonideality into the intracellular solution. Compared with the ideal solution model based on the modified Mazur’s water transport equation, the new model further coupled Fahy’s water transport equation, ice nucleation, and the diffusion-limited ice growth theory. The new model was then used to study the freezing process of mouse oocyte in the presence of glycerol as a cryoprotective agent. Through the comparative analysis of the influence of cooling rate and initial glycerol concentration on the intracellular solution volume and the crystallized volume fraction under these two models, it was found that the predictions by these two models had significant difference at low cooling rate, while they are consistent at high cooling rate.

Key words: intracellular ice formation, vitrification, nonideal solution