Synthesis of Worm-like Mesoporous Silica and Enzymatic Activity of the Immobilized Laccase

doi:10.6023/A12100836

Acta Chimica Sinica ›› 2013, Vol. 71 ›› Issue (04): 602-612.DOI: 10.6023/A12100836 Previous Articles Next Articles

Article

蠕虫状孔道介孔SiO₂的合成及其固定化漆酶的活性

张剑波^a, 杨宇翔^b, 田美娟^b, 邓常健^b, 曹磊^b, 袁宏明^c

a 北京大学环境科学与工程学院北京 100871;
b 华东理工大学化学与分子工程学院上海 200237;
c 无机合成与制备化学国家重点实验室吉林大学化学学院长春 130012

投稿日期:2012-11-08 发布日期:2013-02-01
通讯作者: 杨宇翔 E-mail:yxyang@ecust.edu.cn

Synthesis of Worm-like Mesoporous Silica and Enzymatic Activity of the Immobilized Laccase

Zhang Jianbo^a, Yang Yuxiang^b, Tian Meijuan^b, Deng Changjian^b, Cao Lei^b, Yuan Hongming^c

a Department of Environmental Sciences, Peking University, Beijing 100871, China;
b School of Chemistry and Molecular Engineering, East China University of Science & Technology,Shanghai 200237, China;
c State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry,Jilin University, Changchun 130012, China

Received:2012-11-08 Published:2013-02-01

Mesoporous silica with worm-like pore channels was synthesized using mixed P123 (polyethyleneoxide-polypropylene oxide-polyethylene oxide triblock copolymer EO₂₀PO₇₀EO₂₀) and ionic cosurfactant {CTAB, SDS or N-myristoyl-D-alanine (C₁₄-D-A)} as a structure-directing agent, and the pore-expanding effect of different amount of PEG-400 on the worm-like pore channels was studied. The synthesized products were characterized using X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and nitrogen sorption analysis. Moreover, the worm-like pore-expanded mesoporous silica was extracted with solvent, and the obtained quaternary ammonium functionalized mesoporous silica with different pore size was applied to immobilize laccase respectively. The stability of immobilized laccase, the optimum pH value acquired and reusability for the degradation of 2,4-DCP were also studied. Through comparative studies, it is found that, matching of sizes between the enzyme molecule and the pore diameter of the mesoporous silica, is essential for specific activity of immobilized laccase and degradation efficiency of 2,4-DCP by immobilized laccase.

Key words: worm-like mesoporous silica, pore-expanding, 2,4-DCP, degradation efficiency, reusability

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Zhang Jianbo, Yang Yuxiang, Tian Meijuan, Deng Changjian, Cao Lei, Yuan Hongming. Synthesis of Worm-like Mesoporous Silica and Enzymatic Activity of the Immobilized Laccase[J]. Acta Chimica Sinica, 2013, 71(04): 602-612.

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蠕虫状孔道介孔SiO₂的合成及其固定化漆酶的活性

Synthesis of Worm-like Mesoporous Silica and Enzymatic Activity of the Immobilized Laccase

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蠕虫状孔道介孔SiO2的合成及其固定化漆酶的活性

Synthesis of Worm-like Mesoporous Silica and Enzymatic Activity of the Immobilized Laccase

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蠕虫状孔道介孔SiO₂的合成及其固定化漆酶的活性