Acta Chimica Sinica ›› 2013, Vol. 71 ›› Issue (04): 602-612.DOI: 10.6023/A12100836 Previous Articles     Next Articles



张剑波a, 杨宇翔b, 田美娟b, 邓常健b, 曹磊b, 袁宏明c   

  1. a 北京大学环境科学与工程学院 北京 100871;
    b 华东理工大学化学与分子工程学院 上海 200237;
    c 无机合成与制备化学国家重点实验室 吉林大学化学学院 长春 130012
  • 投稿日期:2012-11-08 发布日期:2013-02-01
  • 通讯作者: 杨宇翔

Synthesis of Worm-like Mesoporous Silica and Enzymatic Activity of the Immobilized Laccase

Zhang Jianboa, Yang Yuxiangb, Tian Meijuanb, Deng Changjianb, Cao Leib, Yuan Hongmingc   

  1. a Department of Environmental Sciences, Peking University, Beijing 100871, China;
    b School of Chemistry and Molecular Engineering, East China University of Science & Technology,Shanghai 200237, China;
    c State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry,Jilin University, Changchun 130012, China
  • Received:2012-11-08 Published:2013-02-01

Mesoporous silica with worm-like pore channels was synthesized using mixed P123 (polyethyleneoxide-polypropylene oxide-polyethylene oxide triblock copolymer EO20PO70EO20) and ionic cosurfactant {CTAB, SDS or N-myristoyl-D-alanine (C14-D-A)} as a structure-directing agent, and the pore-expanding effect of different amount of PEG-400 on the worm-like pore channels was studied. The synthesized products were characterized using X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and nitrogen sorption analysis. Moreover, the worm-like pore-expanded mesoporous silica was extracted with solvent, and the obtained quaternary ammonium functionalized mesoporous silica with different pore size was applied to immobilize laccase respectively. The stability of immobilized laccase, the optimum pH value acquired and reusability for the degradation of 2,4-DCP were also studied. Through comparative studies, it is found that, matching of sizes between the enzyme molecule and the pore diameter of the mesoporous silica, is essential for specific activity of immobilized laccase and degradation efficiency of 2,4-DCP by immobilized laccase.

Key words: worm-like mesoporous silica, pore-expanding, 2,4-DCP, degradation efficiency, reusability