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Acta Chimica Sinica ›› 2004, Vol. 62 ›› Issue (15): 1379-1384. Previous Articles     Next Articles

聚丙烯片基不同气氛下等离子体改性及DNA原位合成研究

汤建新1,2, 周灵君3, 陈洪1, 何农跃1,2,4   

  1. 1. 株洲工学院包装新材料与技术重点实验室, 株洲, 412008;
    2. 东南大学教育部分子与生物分子电子学重点实验室, 南京, 210096;
    3. 南京中医药大学药学院, 南京, 210029;
    4. 南京医科大学第一附属医院, 南京, 210029
  • 投稿日期:2004-01-13 修回日期:2004-04-02 发布日期:2014-02-17
  • 通讯作者: 何农跃,E-mail:nyhe@seu.edu.cn;Fax:025-83619983. E-mail:nyhe@seu.edu.cn
  • 基金资助:
    国家863计划(Nos.102-01-01,2003AA302270),国家自然科学基金(No.60071001),教育部跨世纪优秀人才培养计划基金(教财司函[2003]36号)和中国博士后科学基金(2002)资助项目.

A Comparative Study on in situ Synthesis of Oligonucleotide on Different Plasma Modified Polypropylene Surfaces

TANG Jian-Xin1,2, ZHOU Ling-Jun3, CHEN Hong1, HE Nong-Yue1,2,4   

  1. 1. Key Laboratory for Packing Materials and New Technology of Zhuzhou Institute of Technology, Zhuzhou 412008;
    2. Key Laboratory for Molecular and Biomolecular Electronics of Ministry of Education, Southeast University, Nanjing 210096;
    3. College of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing 210029;
    4. First Affiliated Hospital, Nanjing Medical University, Nanjing 210029
  • Received:2004-01-13 Revised:2004-04-02 Published:2014-02-17

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This paper compared results of oligonucleotide probe array in-situ synthesized on the hydrogen and nitrogen plasma modified polypropylene (PP) surface with those on the amino plasma modified PP surface and also with those on oxygen plasma and silanizing modified PP surface.X-ray photoelectron spectroscopy (XPS) analysis confirmed the presence of polar amino groups on the oxygen plasma and silanizing treated PP surfaces.The specificity of the probe arrays was discriminated by hybridizing with fluorescent target sequences.The results indicated that the three different plasma-functionalized PP surfaces all could serve as good substrates for high-density oligonucleotide array synthesis and DNA chip fabrication.However, the hydrogen and nitrogen plasma-functionalized PP surface was the best.

Key words: plasma, polypropylene, oligonucleotide probe array, in-situ synthesis