Chin. J. Org. Chem. ›› 2016, Vol. 36 ›› Issue (11): 2763-2768.DOI: 10.6023/cjoc201605013 Previous Articles     Next Articles



管超建, 王涛, 王君, 李宜明   

  1. 合肥工业大学生物与医学工程学院 合肥 230009
  • 收稿日期:2016-05-10 修回日期:2016-06-08 发布日期:2016-07-07
  • 通讯作者: 王君, 李宜明;
  • 基金资助:


Synthesis of Post-translational Modifier Protein NEDD8 via Ligation of Peptide Hydrazides

Guan Chaojian, Wang Tao, Wang Jun, Li Yiming   

  1. Department of Pharmacy, School of Biological and Medical Engineering, Hefei University of Technology, Hefei 230009
  • Received:2016-05-10 Revised:2016-06-08 Published:2016-07-07
  • Supported by:

    Project supported by the National Natural Science Foundation of China (Nos.21372058,21572043).

As an important ubiquitin-like modifier protein in eukaryotic organisms, NEDD8 (neural precursor cell expressed developmentally down-regulated 8) is involved in regulating a series of important life processes in cells. Nowadays, the major method for obtaining NEDD8 is recombinant protein expression. However, the yield is relatively low and the recombined tag for purification needs to be removed in an extra step. In present work, NEDD8 protein was first synthesized in homogeneity by using high temperature assisted solid-phase peptide synthesis (SPPS) combining with the one-pot ligation-desulfurization strategy. This method lays the foundation for the study of NEDD8 modified proteins in future.

Key words: NEDD8, high temperature assisted SPPS, ligation of peptide hydrazide, one-pot ligation-desulfurization