from methanol extracts of the whole plants of Anemone tetrasepala royle, a new triterpenoid saponin, named tetrasepaloside (1), along with oleanolic acid 3-O-α glucopyranosyl (1→ 2)-α - arabinoside (2), HN-saponin F (3), pulsatiloside C (4), akebia saponin D (5), cauloside D (6), ciwujianoside A1(7) and cauloside F (8), was isolated. By means of chemical degradation and spectral analysis, including 2D-NMR spectroscopic methods (1^H-1^HCOSY, HMBC and HMQC), the structure of 1 was elucidated as 23-O-(2', 3'-monoacetonide)- α - ribofuranosyl-hederagenin-28-O- - rhamnopyranosyl(1→ 4)-β - glucopyranosyl(1→ 6)-β - gulcopyranoside. All these compounds were reported for the first time from this plant.

Key words: CHEMICAL COMPOSITION, TRITERPENE, ANEMONE