Detection for Polyketide Synthase Gene of Aspergillus ochraceus based on Au Nanoparticles Enhancing SPR Biosensor

doi:10.6023/A1201033

Acta Chimica Sinica ›› 2012, Vol. 70 ›› Issue (11): 1304-1308.DOI: 10.6023/A1201033 Previous Articles Next Articles

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纳米金增强SPR 检测产毒赭曲霉中PKS 基因的特异性碱基

李迎, 林钊, 李蓉卓, 刘霞

湖南农业大学食品科技学院食品科学与生物技术湖南省重点实验室长沙 410128

投稿日期:2012-01-03 修回日期:2012-03-06 发布日期:2012-03-09
通讯作者: 刘霞 E-mail:liuxiaspr@gmail.com
基金资助:
湖南省教育厅优秀青年项目(No. 10B050)、长沙市科技计划重点项目(No. K1005181-21)和湖南省研究生创新基金(No. CX2010B281)资助项目.

Detection for Polyketide Synthase Gene of Aspergillus ochraceus based on Au Nanoparticles Enhancing SPR Biosensor

Li Ying, Lin Zhao, Li Rongzhuo, Liu Xia

College of Food Science and Technology, Hunan Agricultural University, Hunan Province Key Laboratory of Food Science and Biotechnology, Changsha 410128

Received:2012-01-03 Revised:2012-03-06 Published:2012-03-09
Supported by:
Project supported by the Scientific Research Fund of Hunan Provincial Education Department (No. 10B050), Key Project of Science and Technology Plan of Changsha (No. K1005181-21) and Graduate Innovation Fund of Hunan Province (No. CX2010B281).

The surface plasmon resonance (SPR) method is established for indirect detection of the ochratoxin A. The 25-base oligonucleotide sequence of polyketide synthase (PKS) gene which is expressed during early stage of ochratoxin A synthesis, was detected respectively using direct assay and Au nanoparticles as sensing membrane assay based on the two channels surface plasmon resonance (SPR) biosensor. At the same time, influence of 6-mercapto-1-hexanol (MCH) blocking on SPR signal was also studied. As a result, the limit of detection (LOD) of the direct assay was 12.5 nmol/L. And the SPR signal was enhanced with 6-mercapto-1-hexanol (MCH) blocking. With Au nanoparticles as a sensing membrane, LOD was 0.25 nmol/L, sensitivity was 50 times higher than that of direct detection. SPR signal could be dramatically enhanced with Au nanoparticles as sensing membrane instead of as label, and the operation was simple.

Key words: surface plasmon resonance (SPR), Au nanoparticles, ochratoxin A, polyketide synthase (PKS)

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Li Ying, Lin Zhao, Li Rongzhuo, Liu Xia. Detection for Polyketide Synthase Gene of Aspergillus ochraceus based on Au Nanoparticles Enhancing SPR Biosensor[J]. Acta Chimica Sinica, 2012, 70(11): 1304-1308.

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纳米金增强SPR 检测产毒赭曲霉中PKS 基因的特异性碱基

Detection for Polyketide Synthase Gene of Aspergillus ochraceus based on Au Nanoparticles Enhancing SPR Biosensor

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