A Symmetrically Split G-quadruplex DNAzymes Biosensor Based on Magnetic Nanoparticles for the Rapid Detection of Hg2+

doi:10.6023/A14030234

Acta Chimica Sinica ›› 2014, Vol. 72 ›› Issue (9): 1029-1035.DOI: 10.6023/A14030234 Previous Articles Next Articles

Article

基于磁纳米颗粒的二段对称分裂式G-四分体DNA酶生物传感器用于Hg²⁺的快速检测

张佳佳, 代佩卿, 李超, 李南忘, 程圭芳, 何品刚, 方禹之

华东师范大学化学系上海 200241

投稿日期:2014-03-31 发布日期:2014-08-27
通讯作者: 程圭芳 E-mail:gfcheng@chem.ecnu.edu.cn
基金资助:
项目受国家自然科学基金（No. 21175045）和国家重大科学仪器设备开发专项（No. 2011YQ150078）资助.

A Symmetrically Split G-quadruplex DNAzymes Biosensor Based on Magnetic Nanoparticles for the Rapid Detection of Hg²⁺

Zhang Jiajia, Dai Peiqing, Li Chao, Li Nanwang, Cheng Guifang, He Pingang, Fang Yuzhi

Chemistry Department, East China Normal University, Shanghai 200241

Received:2014-03-31 Published:2014-08-27
Supported by:
Project supported by the National Natural Science Foundation of China (No. 21175045) and National Key Foundation for Exploring Scientific Instrument (No. 2011YQ150078).

1. A Symmetrically Split G-quadruplex DNAzymes Biosensor Based on Magnetic Nanoparticles for the Rapid Detection of Hg²⁺.PDF(392KB)

Abstract

A biosensor composed of magnetic nanoparticles and symmetrically split G-quadruplex DNAzymes was proposed for the rapid and sensitive detection of Hg²⁺. It was mainly based on the formation of a special thymine-Hg²⁺-thymine (T-Hg-T) structure by T-riched nucleic acid sequences and Hg²⁺, and then G-quadruplex DNAzymes catalyzed the oxidation of H₂O₂ and 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS), so that the trace amount of Hg²⁺ in water can be successfully detected by colorimetry. The assembly of the biosensor was characterized by using of UV-Visible spectroscopy, CD spectroscopy and fluorescence microscopy imaging techniques respectively. The magnetic nanoparticles coated with T-riched short oligonucleotide (DNA1) via streptavidin-biodin interaction were employed as the capture elements. The long oligonucleotides (DNA2) consisting of the T-riched sequence in the middle and two G-riched fragments at the both ends were used as the sensing elements. The procedure of the protocol was divided into three steps. First: the magnetic nanoparticles covered with DNA1 (MNPs/DNA1) mixed with DNA2 were added into a water sample to capture the target Hg²⁺ by forming the special T-Hg-T structure. Incubating 2 h at 37 ℃, two G-riched fragments at the ends of DNA2 were close to each other assembling the symmetrically G-quadruplex DNAzymes in the presence of hemin. Then, the supernatant was discarded by magnetic separation, and the residues were washed several times to resuspend in the mixture solution containing definite amount of H₂O₂ and ABTS. The catalytic signal of absorbance of ABTS⁺ was recorded by UV-Visible spectrophotometer. The utilization of MNPs can greatly reduce the background signal, and the employment of symmetrically split DNAzymes made the design of the experiment more flexible and selectable. The biosensor was sensitive, and the calibration curve was identified in the range from 0.8 nmol/L to 20 nmol/L with a detection limit of 0.3 nmol/L. And the biosensor can detect Hg²⁺ without interference in the presence of a large number of competing ions. This sensor was simple, inexpensive, and renewable. Satisfactory values between 95.3% and 104.4% were obtained for the recovery experiments, and it can meet the requirement of detection of trace Hg²⁺ in natural water especially in drinking water.

Key words: magnetic nanoparticles, symmetrically split G-quadruplex, DNAzymes, biosensors, colorimetry, mercury ions

Cite this article

Zhang Jiajia, Dai Peiqing, Li Chao, Li Nanwang, Cheng Guifang, He Pingang, Fang Yuzhi. A Symmetrically Split G-quadruplex DNAzymes Biosensor Based on Magnetic Nanoparticles for the Rapid Detection of Hg²⁺[J]. Acta Chimica Sinica, 2014, 72(9): 1029-1035.

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基于磁纳米颗粒的二段对称分裂式G-四分体DNA酶生物传感器用于Hg²⁺的快速检测

A Symmetrically Split G-quadruplex DNAzymes Biosensor Based on Magnetic Nanoparticles for the Rapid Detection of Hg²⁺

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基于磁纳米颗粒的二段对称分裂式G-四分体DNA酶生物传感器用于Hg2+的快速检测