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Acta Chimica Sinica ›› 1993, Vol. 51 ›› Issue (7): 632-638. Previous Articles Next Articles
Original Articles
穆绍林;阚锦晴
发布日期:
MU SHAOLIN;KAN JINQING
Published:
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The polypyrrole uricase electrode has a fast bioelectrochem. response. In the region from 5.9?0-6 to 1.5?0-3 mol譫m-3 of uric acid, the response current of the polypyrrole uricase electrode is proportional to the substrate concentration The apparent activation energy of the catalyzed reaction of the immobilized enzyme is 34.81 kL譵ol-1. As compared with the soluble uricase, advantageous changes were obtained for the immobilized enzyme in the pH and temperature dependences of the activity. The repeatability of the uricase electrode for the determination of the uric acid concentration is affected by the kind of conducting polymer, which is used for a support material of the immobilized enzyme.
Key words: BIOLOGICAL ACTIVITY, URATE OXIDASE, BIOELECTROCHEMISTRY, ENZYME ELECTRODES, POLYPYRROLE
CLC Number:
O646
MU SHAOLIN;KAN JINQING. Bioelectrochemical activities of the polypyrrole uricase electrode[J]. Acta Chimica Sinica, 1993, 51(7): 632-638.
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