In this paper, a new immunoassay technique, flow Chemiluminescence injection immunoassay, in which controlleld pore glass immobilized with antibody was used as immunoreactor and chemiluminescence for end-point monitoring, has been developed. Researches show that the time required per assay only 20 min with this method while 20h with conventional ELISA. Moreover, the whole assay can been taken automatically under the control of a microprocessor. This technique has been used for the determination of HBsAg in serum, and the results are comparable with that of ELISA. In addition, it has a relative standard deviation of 7.2%(9 assays). So this technique offers advantages of automatically rapidly and stablity.

Key words: HEPATITIS, SERUM, FLOW INJECTION ANALYSIS, CHEMILUMINESSENCE, SURFACE ANTIGENS